概述

  • 产品名称Anti-TCEB2抗体
    参阅全部 TCEB2 一抗
  • 描述
    兔多克隆抗体to TCEB2
  • 特异性This antibody detects TCEB2 only.
  • 经测试应用适用于: IP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein expressed in E. coli.

性能

应用

Our Abpromise guarantee covers the use of ab45297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
ICC/IF
WB
  • 应用说明IP: Use at an assay dependent dilution.
    WB: 1/1000. Predicted molecular weight: 18 kDa.
    ICC/IF: Use at a concentration of 1-5 µg/ml.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
      The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Contains 1 ubiquitin-like domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EloB antibody
      • ELOB_HUMAN antibody
      • Elongin 18 kDa subunit antibody
      • Elongin B antibody
      • Elongin-B antibody
      • RNA polymerase II transcription factor SIII p18 subunit antibody
      • RNA polymerase II transcription factor SIII subunit B antibody
      • SIII antibody
      • SIII p18 antibody
      • TCEB 2 antibody
      • Tceb2 antibody
      • Transcription elongation factor B (SIII) polypeptide 2 antibody
      • Transcription elongation factor B polypeptide 2 antibody
      see all

    Anti-TCEB2 antibody 图像

    • All lanes : Anti-TCEB2 antibody (ab45297) at 1/1000 dilution

      Lane 1 : Recombinant TCEB2 protein (15ug/lane)
      Lane 2 : Negative control protein


      Predicted band size : 18 kDa
      Observed band size : 18 kDa
    • All lanes : Anti-TCEB2 antibody (ab45297) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251) at 20 µg
      Lane 3 : Skeletal Muscle Normal (Human) Tissue Lysate (ab15370) at 20 µg
      Lane 4 : Negative control - Small Intestine (Human) Tissue Lysate (ab7923)at 20 µg

      Secondary
      Rabbit IgG secondary antibody (ab28446) at 1/5000 dilution

      Predicted band size : 18 kDa
      Observed band size : 18 kDa
      Additional bands at : 100 kDa,13 kDa,30 kDa,32 kDa. We are unsure as to the identity of these extra bands.
    • ICC/IF image of ab45297 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45297, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-TCEB2 antibody (ab45297)参考文献

    ab45297 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab45297.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"