概述

  • 产品名称Anti-TCEAL1抗体
    参阅全部 TCEAL1 一抗
  • 描述
    小鼠单克隆抗体to TCEAL1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human, Recombinant Fragment
  • 免疫原

    Recombinant fragment: MDKPRKENEE EPQSAPKTDE ERPPVEHSPE KQSPEEQSSE EQSSEEEFFP EELLPELLPE MLLSEERPPQ EGLSRKDLFE GRPPMEQPPC GVGKHKLEEG , corresponding to amino acids 1-100 of Human TCEAL1 with tag (NP_001006640)

  • 阳性对照
    • TCEAL1 transfected 293T cell lysate; Recombinant fragment of human TCEAL1.

性能

应用

Our Abpromise guarantee covers the use of ab77661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 18 kDa).
ELISA Use at an assay dependent dilution.

靶标

  • 功能May be involved in transcriptional regulation. Modulates various viral and cellular promoters in a promoter context-dependent manner. For example, transcription from the FOS promoter is increased, while Rous sarcoma virus (RSV) long terminal repeat (LTR) promoter activity is repressed. Does not bind DNA directly.
  • 组织特异性Expressed in all tissues examined. Highly expressed in heart, ovary, prostate and skeletal muscle. Moderately expressed in brain, placenta, testis and small intestine. Weakly expressed in lung, liver and spleen. Expressed in several cancer cell lines.
  • 序列相似性Belongs to the TFS-II family. TFA subfamily.
  • 翻译后修饰Phosphorylation of Ser-36 and Ser-37 is critical for transcriptional repression.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Highly similar to gene pp21 protein [H.sapiens] antibody
    • Nuclear phosphoprotein p21/SIIR antibody
    • p21 antibody
    • pp21 antibody
    • SIIR antibody
    • TCAL1_HUMAN antibody
    • TCEA like protein 1 antibody
    • TCEA-like protein 1 antibody
    • TCEAL1 antibody
    • Transcription elongation factor A (SII) like 1 antibody
    • Transcription elongation factor A protein like 1 antibody
    • Transcription elongation factor A protein-like 1 antibody
    • Transcription elongation factor S II protein like 1 antibody
    • Transcription elongation factor S-II protein-like 1 antibody
    see all

Anti-TCEAL1 antibody 图像

  • Anti-TCEAL1 antibody (ab77661) at 5 µg/ml + Recombinant fragment of human TCEAL1 at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 18 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TCEAL1 antibody (ab77661) at 5 µg/ml

    Lane 1 : TCEAL1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 18 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

Anti-TCEAL1 antibody (ab77661)参考文献

ab77661 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77661.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"