重组
RabMAb

Anti-TC10抗体[Y304] (ab32079)

概述

  • 产品名称
    Anti-TC10抗体[Y304]
    参阅全部 TC10 一抗
  • 描述
    兔单克隆抗体[Y304] to TC10
  • 特异性
    ab32079 recognises TC10.
  • 经测试应用
    适用于: WB, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to the third GTP unit of human TC10

  • 阳性对照
    • 293 cell lysate, HeLa cells
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab32079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.Can be blocked with TC10 peptide (ab186757).
ICC/IF 1/250 - 1/500.
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能
      Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia.
    • 序列相似性
      Belongs to the small GTPase superfamily. Rho family.
    • 翻译后修饰
      May be post-translationally modified by both palmitoylation and polyisoprenylation.
    • 细胞定位
      Cytoplasm. Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ARHQ antibody
      • Ras homolog gene family member Q antibody
      • RAS like family 7 member A antibody
      • Ras like protein family member 7A antibody
      • Ras like protein TC10 antibody
      • Ras related GTP binding protein TC10 antibody
      • Ras-like protein family member 7A antibody
      • Ras-like protein TC10 antibody
      • RASL7A antibody
      • Rho related GTP binding protein RhoQ antibody
      • Rho-related GTP-binding protein RhoQ antibody
      • Rhoq antibody
      • RHOQ_HUMAN antibody
      • TC10A antibody
      see all

    图片

    • Anti-TC10 antibody [Y304] (ab32079) at 1/1000 dilution + 293 cell lysate

      Predicted band size : 23 kDa
      Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent analysis of TC10 expression in HeLa cells using 1/250 ab 32079.

    文献

    This product has been referenced in:
    • Fujita A  et al. GTP hydrolysis of TC10 promotes neurite outgrowth through exocytic fusion of Rab11- and L1-containing vesicles by releasing exocyst component Exo70. PLoS One 8:e79689 (2013). ICC/IF . Read more (PubMed: 24223996) »
    • Heasman SJ  et al. Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration. J Cell Biol 190:553-63 (2010). WB ; Human . Read more (PubMed: 20733052) »

    See all 4 Publications for this product

    客户评价及客户问答

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - other (embryonic dorsal root ganglia)
    Loading amount
    30 µg
    Specification
    embryonic dorsal root ganglia
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
    Username

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    Verified customer

    提交于 Nov 03 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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