概述

  • 产品名称Anti-Tbx6抗体
    参阅全部 Tbx6 一抗
  • 描述
    小鼠单克隆抗体to Tbx6
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 191-300 of Human Tbx6

性能

应用

Our Abpromise guarantee covers the use of ab58255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47 kDa.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 35 µg/ml.

靶标

  • 功能Probable transcriptional regulator involved in developmental processes. Could be required for specification of paraxial mesoderm structures during gastrulation.
  • 组织特异性Expressed in fetal tail bud, posterior spinal tissue, intervertebral disk and testis. Also expressed in adult testis, kidney, lung, muscle and thymus.
  • 序列相似性Contains 1 T-box DNA-binding domain.
  • 发展阶段Expressed during gastrulation and during a second phase in some adult tissues.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DFNB 67 antibody
    • DFNB67 antibody
    • SCDO5 antibody
    • T box 6 antibody
    • T box protein 6 antibody
    • T box transcription factor TBX 6 antibody
    • T box transcription factor TBX6 antibody
    • T-box protein 6 antibody
    • T-box transcription factor TBX6 antibody
    • Tbox 6 antibody
    • TBX 6 antibody
    • tbx6 antibody
    • TBX6_HUMAN antibody
    see all

Anti-Tbx6 antibody 图像

  • Overlay histogram showing HEK293 cells stained with ab58255 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58255, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


  • Predicted band size : 47 kDa
    Tbx6 antibody (ab58255) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab58255 at 35 ug/ml staining Tbx6 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

Anti-Tbx6 antibody (ab58255)参考文献

ab58255 has not yet been referenced specifically in any publications.

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