概述

  • 产品名称
  • 描述
    兔多克隆抗体to Tbx6
  • 经测试应用
    适用于: IHC-Fr, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Tbx6.

    (Peptide available as ab26243.)

  • 阳性对照
    • This antibody gave a positive signal in human and mouse testis and lung tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab38883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 25157815
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).

靶标

  • 功能
    Probable transcriptional regulator involved in developmental processes. Could be required for specification of paraxial mesoderm structures during gastrulation.
  • 组织特异性
    Expressed in fetal tail bud, posterior spinal tissue, intervertebral disk and testis. Also expressed in adult testis, kidney, lung, muscle and thymus.
  • 序列相似性
    Contains 1 T-box DNA-binding domain.
  • 发展阶段
    Expressed during gastrulation and during a second phase in some adult tissues.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DFNB 67 antibody
    • DFNB67 antibody
    • SCDO5 antibody
    • T box 6 antibody
    • T box protein 6 antibody
    • T box transcription factor TBX 6 antibody
    • T box transcription factor TBX6 antibody
    • T-box protein 6 antibody
    • T-box transcription factor TBX6 antibody
    • Tbox 6 antibody
    • TBX 6 antibody
    • tbx6 antibody
    • TBX6_HUMAN antibody
    see all

图片

  • All lanes : Anti-Tbx6 antibody (ab38883) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate
    Lane 2 : Lung (Mouse) Tissue Lysate
    Lane 3 : Human testis tissue lysate - total protein (ab30257)
    Lane 4 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • IHC-P image of Tbx6 staining with ab38883 on tissue sections from an E8.5 mouse embryo. The sections were Paraformaldehyde fixed, and permeabilized in PBST (PBS/0.5% v/v TritonX100), before incubation with ab38883 (1/500 dilution) for 48 hours at 4°C in 1% BSA, 5% donkey serum in PBST. The secondary used was an Alexa-Fluor 555 conjugated donkey anti-rabbit polyclonal, at a 1/1000 dilution.

    See Abreview

文献

This product has been referenced in:
  • Chal J  et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Nat Protoc 11:1833-50 (2016). Read more (PubMed: 27583644) »
  • Gouti M  et al. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol 12:e1001937 (2014). ICC/IF ; Mouse . Read more (PubMed: 25157815) »

See all 2 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Paraxial mesoderm)
Permeabilization
Yes - PBS/0.1% v/v TritonX100 (Sigma)
Specification
Paraxial mesoderm
Blocking step
FBS as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 13 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E8.5 mouse embryo section)
Specification
E8.5 mouse embryo section
Fixative
Paraformaldehyde
Permeabilization
Yes - PBST (PBS/0.5% v/v TritonX100 (Sigma)
Blocking step
(agent) for 30 minute(s) · Concentration: 100%
Username

Abcam user community

Verified customer

提交于 Aug 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (tail bud e12.5)
Loading amount
5 µg
Specification
tail bud e12.5
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Aug 15 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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