Anti-TBX5抗体(ab49308)

概述

  • 产品名称Anti-TBX5抗体
    参阅全部 TBX5 一抗
  • 描述
    兔多克隆抗体to TBX5
  • 特异性This antibody is specific for TBX5.
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide found within the following sequence: NSMHKYQPRL HIVKADENNG FGSKNTAFCT HVFPETAFIA VTSYQNHKIT (Human)

  • 阳性对照
    • HepG2 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab49308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-TBX5 antibody 图像

  • Lane 1 : Marker
    Lane 2 : Anti-TBX5 antibody (ab49308) at 5 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg/ml

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 53 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of Human Brain, cortex tissue at an antibody concentration of 5.0µg/ml using anti-TBX5 antibody (ab49308)

Anti-TBX5 antibody (ab49308)参考文献

ab49308 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification Human pluripotent stem cell derived cardiomyocyte
Permeabilization Yes - saponin
Fixative Paraformaldehyde
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提交于 Aug 27 2014

Thank you for your question. The peptide immunogen for ab49308 has 100% homology to the Scyliorhinus canicula T-box protein 5 (Tbx5) sequence you provided. I hope this is helpful. Please contact me again if you have any further questions.

Thank you for your enquiry. Thus far I have found the following information regarding potential reactivity of our TBX5 antibodies to Scyliorhinus canicula: ab58253 - no chance for cross reactivity ab86370 - region of immunogen overlaps partially but th...

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Please find below two links to our recommended protocols: One details a full nuclear extraction protocol http://www.abcam.com/ps/pdf/protocols/Nuclear%20fractionation%20protocol.pdf one details the extraction using RIPA buffer:http://www.abcam...

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Thank you for your enquiry. I am sorry to hear you are having a problem with ab49308. As you mention, this antibody has so far not been tested in zebrafish but we would expect the antibody to work in this species due to the homology of the protein i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"