概述

  • 产品名称
  • 描述
    兔多克隆抗体to TBX5
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse TBX5.

  • 阳性对照
    • This antibody gave a positive signal in Mouse embryo brain (E10)tissue lysate, as well as F9 and NIH3T3 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab101227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 24797668
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 57 kDa).

靶标

  • 功能
    Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation.
  • 疾病相关
    Defects in TBX5 are the cause of Holt-Oram syndrome (HOS) [MIM:142900]. HOS is a developmental disorder affecting the heart and upper limbs. It is characterized by thumb anomaly and atrial septal defects.
  • 序列相似性
    Contains 1 T-box DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Holt Oram syndrome antibody
    • HOS antibody
    • T box 5 antibody
    • T box protein 5 antibody
    • T box transcription factor TBX 5 antibody
    • T box transcription factor TBX5 antibody
    • T-box protein 5 antibody
    • T-box transcription factor TBX5 antibody
    • TBX 5 antibody
    • TBX5 antibody
    • TBX5_HUMAN antibody
    • Transcription factor T box 5 antibody
    see all

图片

  • All lanes : Anti-TBX5 antibody (ab101227) at 1 µg/ml

    Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : E10 Mouse Embryo Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

文献

This product has been referenced in:
  • Jiang XY  et al. Inhibition of Gata4 and Tbx5 by Nicotine-Mediated DNA Methylation in Myocardial Differentiation. Stem Cell Reports 8:290-304 (2017). WB, IF, Flow Cyt . Read more (PubMed: 28111280) »
  • Malliaras K  et al. Stimulation of endogenous cardioblasts by exogenous cell therapy after myocardial infarction. EMBO Mol Med 6:760-77 (2014). ICC/IF ; Mouse . Read more (PubMed: 24797668) »

See all 2 Publications for this product

客户评价及客户问答

Thank you for your enquiry. Thus far I have found the following information regarding potential reactivity of our TBX5 antibodies to Scyliorhinus canicula: ab58253 - no chance for cross reactivity ab86370 - region of immunogen overlaps partially but th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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