概述

  • 产品名称Anti-Tbx3抗体
    参阅全部 Tbx3 一抗
  • 描述
    兔多克隆抗体to Tbx3
  • 特异性Ab66306 is specific for isoform 3 of Tbx3.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Dog
  • 免疫原

    A region within synthetic peptide: CRDKGSPAVK AHLFAAERPR DSGRLDKASP DSRHSPATIS SSTRGLGAEE, corresponding to internal sequence amino acids 404-453 of Human Tbx3

  • 阳性对照
    • HepG2 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab66306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21341990
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 66 kDa. Specific for isoform 3 of Tbx3. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-Tbx3 antibody 图像

  • Anti-Tbx3 antibody (ab66306) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa


  • Predicted band size : 66 kDa


    Sample Type: Human iPS cells and 293T cell lysate

    Primary Dilution (ab66306): 1.25ug/mL

Anti-Tbx3 antibody (ab66306)参考文献

This product has been referenced in:
  • Yano T  et al. Tara up-regulates E-cadherin transcription by binding to the Trio RhoGEF and inhibiting Rac signaling. J Cell Biol 193:319-32 (2011). Read more (PubMed: 21482718) »
  • Bozyk PD  et al. Mesenchymal stromal cells from neonatal tracheal aspirates demonstrate a pattern of lung-specific gene expression. Stem Cells Dev 20:1995-2007 (2011). ICC/IF ; Human . Read more (PubMed: 21341990) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells (D3 cell line))
Loading amount 400000 cells
Specification mouse embryonic stem cells (D3 cell line)
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Kate Hawkins

Verified customer

提交于 Mar 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"