概述

  • 产品名称
  • 描述
    兔多克隆抗体to Tbx2
  • 经测试应用
    适用于: WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Tbx2.

    (Peptide available as ab35983.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human tissue lysates: Kidney; Lung; Heart

性能

应用

Our Abpromise guarantee covers the use of ab33298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

靶标

Anti-Tbx2 antibody 图像

  • All lanes : Anti-Tbx2 antibody (ab33298) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Lung (Human) Tissue Lysate
    Lane 3 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
  • Formalin-fixed paraffin-embedded mouse bone marrow section stained with ab33298 Tbx2 antibody at 1/500 dilution.
  • ab33298 staining Tbx2 in mouse heart and somatopleure tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with methanol. Samples were then blocked with 5% serum for 2 hours at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An undiluted Cy2®-conjugated Donkey anti-rabbit IgG was used as secondary antibody.

    See Abreview

Anti-Tbx2 antibody (ab33298)参考文献

This product has been referenced in:
  • Liu F  et al. TBX2 expression is regulated by PAX3 in the melanocyte lineage. Pigment Cell Melanoma Res 26:67-77 (2013). Read more (PubMed: 23020925) »
  • Lüdtke TH  et al. Tbx2 controls lung growth by direct repression of the cell cycle inhibitor genes Cdkn1a and Cdkn1b. PLoS Genet 9:e1003189 (2013). Read more (PubMed: 23341776) »

See all 2 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart and somatopleure)
Specification
Heart and somatopleure
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Jun Xu

Verified customer

提交于 May 25 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Bone Marrow)
Specification
Bone Marrow
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Apr 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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