Anti-TBL1抗体- ChIP Grade (ab24548)

概述

  • 产品名称Anti-TBL1抗体- ChIP Grade
    参阅全部 TBL1 一抗
  • 描述
    兔多克隆抗体to TBL1 - ChIP Grade
  • 特异性Does not cross-react with TBLR1.
  • 经测试应用适用于: ChIP, WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human)

  • 阳性对照
    • HeLa nuclear extract

应用

Our Abpromise guarantee covers the use of ab24548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent dilution.
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 63 kDa).
IP Use at an assay dependent dilution.
ICC/IF 1/300.

靶标

  • 功能F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of corepressor complexes that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of transcription repressor complexes, thereby allowing cofactor exchange.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the WD repeat EBI family.
    Contains 1 F-box-like domain.
    Contains 1 LisH domain.
    Contains 8 WD repeats.
  • 结构域The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EBI antibody
    • F box like/WD repeat protein TBL1X antibody
    • F-box-like/WD repeat-containing protein TBL1X antibody
    • SMAP 55 antibody
    • SMAP55 antibody
    • TBL 1 antibody
    • TBL 1X antibody
    • TBL1 antibody
    • TBL1X antibody
    • TBL1X_HUMAN antibody
    • Transducin (beta) like 1 antibody
    • Transducin (beta) like 1 X linked antibody
    • Transducin (beta) like 1X linked antibody
    • Transducin beta like 1 X linked antibody
    • Transducin beta like 1X antibody
    • Transducin beta like 1X protein antibody
    • Transducin beta like protein 1, X linked antibody
    • Transducin beta-like protein 1X antibody
    • Transducin-beta-like protein 1 antibody
    • X-linked antibody
    see all

Anti-TBL1 antibody - ChIP Grade 图像

  • Anti-TBL1 antibody - ChIP Grade (ab24548) at 1/1000 dilution + HeLa nuclear extract.

    Predicted band size : 63 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
  • TBL1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to TBL1 - ChIP Grade (ab24548) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab24548.
    Secondary: Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 64kDa: TBL1.
  • ab24548 staining TBL1 (green) in Human Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 in PBS + 0.2% fish scale gelatin) for 20 minutes at 25°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

Anti-TBL1 antibody - ChIP Grade (ab24548)参考文献

This product has been referenced in:
  • Chen Y  et al. TBLR1 fuses to retinoid acid receptor a in a variant t(3;17)(q26;q21) translocation of acute promyelocytic leukemia. Blood 124:936-45 (2014). Read more (PubMed: 24782508) »
  • Heckman LD  et al. Rett-causing mutations reveal two domains critical for MeCP2 function and for toxicity in MECP2 duplication syndrome mice. Elife 3:N/A (2014). WB ; Mouse . Read more (PubMed: 24970834) »

See all 6 Publications for this product

Product Wall

Application ChIP
Detection step Semiquantitative PCR
Sample Mouse Tissue lysate - whole (Mouse liver)
Specification Mouse liver
Negative control IgG
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Positive control Pol11
Username

Mr. Natarajan Balasubramaniyan

Verified customer

提交于 Sep 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Cell lines)
Loading amount 35 µg
Specification Cell lines
Gel Running Conditions Reduced Denaturing (10 % SDS PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 01 2013

Thank you very much for your call today and for letting us know about the trouble with this antibody.

Please keep me updated about the results after trying the alterations that we discussed. If the results are still not satisfactory, I'll be ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela cells)
Specification Hela cells
Fixative Methanol
Permeabilization No
Blocking step Fish scale gelatin in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"