概述

  • 产品名称
    Anti-TBC1D21抗体
  • 描述
    兔多克隆抗体to TBC1D21
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (TTLSPENSLS ARQSASFILV KRKPPIDKTE WDSFFDESGH LAKSRDFICV) of human TBC1D21 (NP_699187).

  • 阳性对照
    • Human fetal brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab89799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.Can be blocked with TBC1D21 peptide (ab184002). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-TBC1D21 antibody (ab89799) at 1 µg/ml (5% skim milk / PBS buffer) + human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <22 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

This product has been referenced in:
  • Lin YH  et al. RAB10 Interacts with the Male Germ Cell-Specific GTPase-Activating Protein during Mammalian Spermiogenesis. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 28067790) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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