Mouse, Rat, Horse, Cow, Pig
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (TTLSPENSLS ARQSASFILV KRKPPIDKTE WDSFFDESGH LAKSRDFICV) of human TBC1D21 (NP_699187).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.Can be blocked with TBC1D21 peptide (ab184002). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
May act as a GTPase-activating protein for Rab family protein(s).
Contains 1 Rab-GAP TBC domain.
Information by UniProt
- male germ cell-specific expressed, containing a RabGAP domain antibody
- MGC34741 antibody
- MgcRabGAP antibody
Western blot - Anti-TBC1D21 antibody (ab89799)
Anti-TBC1D21 antibody (ab89799) at 1 µg/ml (5% skim milk / PBS buffer) + human fetal brain lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
39 kDaObserved band size:
42 kDa (why is the actual band size different from the predicted?
)Additional bands at:
<22 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
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