The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能Negative regulator of starvation-induced autophagosome formation.
序列相似性Contains 1 Rab-GAP TBC domain.
细胞定位Golgi apparatus, cis-Golgi network. Golgi apparatus, trans-Golgi network. After amino acid starvation, Golgi apparatus-associated protein levels increase compared with fed conditions. May be cycling between the Golgi apparatus and an endosomal pool, redistributing to the Golgi apparatus upon starvation.