antigen sequence, corresponding to amino acids 264-371 of Human TBC1D12.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
May act as a GTPase-activating protein for Rab family protein(s).
Contains 1 Rab-GAP TBC domain.
Information by UniProt
- TBC1 domain family member 12 antibody
- TBC12_HUMAN antibody
- TBC1D12 antibody
Immunocytochemistry/ Immunofluorescence - Anti-TBC1D12 antibody (ab122453)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122453 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D12 antibody (ab122453)
ab122453, at 1/20 dilution, staining TBC1D12 in paraffin embedded Human kidney tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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