概述

  • 产品名称Anti-TAZ抗体
    参阅全部 TAZ 一抗
  • 描述
    兔多克隆抗体to TAZ
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to N terminal amino acids 72-121 (GHPGPRLAGG AQHVRSHSSP ASLQLGTGAG AAGSPAQQHA HLRQQSYDVT) of human TAZ (NP_056287).

  • 阳性对照
    • Hela cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.

性能

应用

Our Abpromise guarantee covers the use of ab84927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
  • 组织特异性Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.
  • 序列相似性Contains 1 WW domain.
  • 结构域The PDZ-binding motif is essential for stimulated gene transcription. It localizes the protein into both punctate nuclear foci and plasma membrane-associated complexes.
    Binds to transcription factors via its WW domain.
  • 翻译后修饰Phosphorylated by LATS2 and STK3/MST2. Phosphorylation by LATS2 results in creation of 14-3-3 binding sites, retention in the cytoplasm, and functional inactivation. Phosphorylation results in the inhibition of transcriptional coactivation through YWHAZ-mediated nuclear export.
  • 细胞定位Nucleus. Cytoplasm. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies. When phosphorylated, is retained in cytoplasm by YWHAZ. Can be retained in the nucleus by MED15.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZP586I1419 antibody
    • FLJ27004 antibody
    • FLJ45718 antibody
    • OTTHUMP00000215994 antibody
    • OTTHUMP00000215995 antibody
    • OTTHUMP00000215996 antibody
    • OTTHUMP00000216001 antibody
    • TAZ antibody
    • Transcriptional co activator with PDZ binding motif antibody
    • Transcriptional coactivator with PDZ binding motif antibody
    • Transcriptional coactivator with PDZ-binding motif antibody
    • WW domain containing transcription regulator 1 antibody
    • WW domain containing transcription regulator protein 1 antibody
    • WW domain-containing transcription regulator protein 1 antibody
    • WWTR 1 antibody
    • WWTR1 antibody
    • WWTR1_HUMAN antibody
    see all

Anti-TAZ antibody 图像



  • Predicted band size : 44 kDa
    Western blot analysis of HepG2 cell lysate labeling TAZ with ab84927 at 1µg/ml.
  • Anti-TAZ antibody (ab84927) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • IHC image of TAZ staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84927, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-TAZ antibody (ab84927)参考文献

This product has been referenced in:
  • Croston TL  et al. Evaluation of the cardiolipin biosynthetic pathway and its interactions in the diabetic heart. Life Sci 93:313-22 (2013). Read more (PubMed: 23872101) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Vascular Smooth Muscle Cell)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification Vascular Smooth Muscle Cell
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 May 16 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (Madine-Darby Canine Kidney cells)
Permeabilization Yes - NP40
Specification Madine-Darby Canine Kidney cells
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 15 2016

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 15 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (N1E-115 murine neuroblastoma)
Specification N1E-115 murine neuroblastoma
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Feb 13 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MDA-MB-231 breast cancer cells)
Loading amount 50000 cells
Specification MDA-MB-231 breast cancer cells
Gel Running Conditions Reduced Denaturing (10% PAGE)
Blocking step 2% milk + 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 05 2012

Thank you for your response.



This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial ofab119373 ((exchange for the original ab84927)) as a free of charge replacement. I hope ...

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Thank you for your enquiry regarding ab84927 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. The protocol looks absolutely fine to me and since after re...

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Gracias por contactarnos. Basándose en la secuencia del péptido usado como inmunógeno, este anticuerpo comparte 100% de homología con la proteína TAZ y tan solo un 65% de homología con YAP1 (isoformas 1-3). Si lo necesitas puedo preguntar por los otros...

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Thank you for your enquiry and your interest in our products.   I have conducted a search for you and for your customer and 4 antibodies come up under TAZ target name using the advanced search engine for primary antibodies:   -  ab118373, -...

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Thank you for contacting us. I am sorry we do not provide discount codes for untested cell lines. The antibodies should be able to detect proteins irrespective of the cells lines if they express targets. I hope this information is helpful t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"