- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
- 产品名称Anti-TAZ抗体
参阅全部 TAZ 一抗 - 描述兔多克隆抗体to TAZ
- 经测试应用适用于: IHC-P, WBmore details
- 种属反应性与反应: Mouse, Human
预测可用于: Rat, Rabbit, Guinea pig, Cow, Dog
- 免疫原
Synthetic peptide corresponding to N terminal amino acids 72-121 (GHPGPRLAGG AQHVRSHSSP ASLQLGTGAG AAGSPAQQHA HLRQQSYDVT) of human TAZ (NP_056287).
- 阳性对照
- Hela cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - 纯度Immunogen affinity purified
- 纯化说明Purified by peptide affinity chromatography method.
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
- Positive Controls
应用
Our Abpromise guarantee covers the use of ab84927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
靶标
- 功能Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
- 组织特异性Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.
- 序列相似性Contains 1 WW domain.
- 结构域The PDZ-binding motif is essential for stimulated gene transcription. It localizes the protein into both punctate nuclear foci and plasma membrane-associated complexes.
Binds to transcription factors via its WW domain. - 翻译后修饰Phosphorylated by LATS2 and STK3/MST2. Phosphorylation by LATS2 results in creation of 14-3-3 binding sites, retention in the cytoplasm, and functional inactivation. Phosphorylation results in the inhibition of transcriptional coactivation through YWHAZ-mediated nuclear export.
- 细胞定位Nucleus. Cytoplasm. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies. When phosphorylated, is retained in cytoplasm by YWHAZ. Can be retained in the nucleus by MED15.
- Information by UniProt
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数据库链接
- Entrez Gene: 25937 Human
- Entrez Gene: 97064 Mouse
- Entrez Gene: 295062 Rat
- Omim: 607392 Human
- SwissProt: Q9GZV5 Human
- SwissProt: Q9EPK5 Mouse
- Unigene: 477921 Human
- Unigene: 535002 Human
see all -
别名
- DKFZP586I1419 antibody
- FLJ27004 antibody
- FLJ45718 antibody
see all
Anti-TAZ antibody 图像
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Western blot - Anti-TAZ antibody (ab84927)All lanes : Anti-TAZ antibody (ab84927) at 1 µg/ml
Lane 1 : HEK293 over-expression
Lane 2 : Empty vector
Lane 3 : Human heart - overexpression
Predicted band size : 44 kDaBlocking buffer used was 3% milk 0.05% TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab84927)IHC image of TAZ staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84927, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry/ Immunofluorescence - Anti-TAZ antibody (ab84927)This image is courtesy of an anonymous Abreview.Immunocytochemical analysis of N1E-115 murine neuroblastoma cell line, labeling TAZ with ab84927. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X100. Blocking was performed with 4% BSA for 30 minutes at 21°C. Incubation with ab84927 diluted 1/100 in 4% BSA for 45 mintues at 21°C.
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Western blot - Anti-TAZ antibody (ab84927)This image is courtesy of an anonymous Abreview.Anti-TAZ antibody (ab84927) at 1/1000 dilution + MDA-MB-231 breast cancer cells at 50000 cells with 2% milk + 1% BSA
Performed under reducing conditions.
Predicted band size : 44 kDaThis image is courtesy of an anonymous Abreview.
Anti-TAZ antibody (ab84927)参考文献
This product has been referenced in:
- Du M et al. MiR-33a suppresses proliferation of NSCLC cells via targeting METTL3 mRNA. Biochem Biophys Res Commun N/A:N/A (2016). WB ; Human . Read more (PubMed: 27856248) »
- Croston TL et al. Evaluation of the cardiolipin biosynthetic pathway and its interactions in the diabetic heart. Life Sci 93:313-22 (2013). Read more (PubMed: 23872101) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"