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Our Abpromise guarantee covers the use of ab84927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
Blocking buffer used was 3% milk 0.05% TBST
IHC image of TAZ staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84927, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemical analysis of N1E-115 murine neuroblastoma cell line, labeling TAZ with ab84927. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X100. Blocking was performed with 4% BSA for 30 minutes at 21°C. Incubation with ab84927 diluted 1/100 in 4% BSA for 45 mintues at 21°C.
This image is courtesy of an anonymous Abreview.