Anti-TAZ抗体(ab119373)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to TAZ
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, surrounding amino acids 386-390 (VESAL) of Human TAZ.

  • 阳性对照
    • Jurkat and HeLa cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab119373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.

靶标

  • 功能
    Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
  • 组织特异性
    Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.
  • 序列相似性
    Contains 1 WW domain.
  • 结构域
    The PDZ-binding motif is essential for stimulated gene transcription. It localizes the protein into both punctate nuclear foci and plasma membrane-associated complexes.
    Binds to transcription factors via its WW domain.
  • 翻译后修饰
    Phosphorylated by LATS2 and STK3/MST2. Phosphorylation by LATS2 results in creation of 14-3-3 binding sites, retention in the cytoplasm, and functional inactivation. Phosphorylation results in the inhibition of transcriptional coactivation through YWHAZ-mediated nuclear export.
  • 细胞定位
    Nucleus. Cytoplasm. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies. When phosphorylated, is retained in cytoplasm by YWHAZ. Can be retained in the nucleus by MED15.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZP586I1419 antibody
    • FLJ27004 antibody
    • FLJ45718 antibody
    • OTTHUMP00000215994 antibody
    • OTTHUMP00000215995 antibody
    • OTTHUMP00000215996 antibody
    • OTTHUMP00000216001 antibody
    • TAZ antibody
    • Transcriptional co activator with PDZ binding motif antibody
    • Transcriptional coactivator with PDZ binding motif antibody
    • Transcriptional coactivator with PDZ-binding motif antibody
    • WW domain containing transcription regulator 1 antibody
    • WW domain containing transcription regulator protein 1 antibody
    • WW domain-containing transcription regulator protein 1 antibody
    • WWTR 1 antibody
    • WWTR1 antibody
    • WWTR1_HUMAN antibody
    see all

Anti-TAZ antibody 图像

  • All lanes : Anti-TAZ antibody (ab119373) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate


    Predicted band size : 44 kDa

实验方案

Anti-TAZ antibody (ab119373)参考文献

ab119373 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (N1E-115 murine neuroblastoma)
Specification
N1E-115 murine neuroblastoma
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

提交于 Feb 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231 breast cancer cell line)
Loading amount
50000 cells
Specification
MDA-MB-231 breast cancer cell line
Gel Running Conditions
Reduced Denaturing (10% PAGE)
Blocking step
2% milk + 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Sep 28 2012

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This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial ofab119373 ((exchange for the original ab84927)) as a free of charge replacement. I hope ...

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Thank you for your enquiry and your interest in our products.   I have conducted a search for you and for your customer and 4 antibodies come up under TAZ target name using the advanced search engine for primary antibodies:   -  ab118373, -...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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