Anti-Taxol抗体(ab26953)

概述

  • 产品名称Anti-Taxol抗体
  • 描述
    兔多克隆抗体to Taxol
  • 经测试应用适用于: ELISAmore details
  • 免疫原

    Taxol conjugation tg (Thyroglobulin bovine).

性能

应用

Our Abpromise guarantee covers the use of ab26953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
  • 应用说明ELISA: 1/10000.
    Antigen is coated on EIA strips at 0.4 µg per well. Antiserum is diluted in series from 1/10 to 1/10,000 and added in separate wells.
    Incubate the antibody for 0.5 hr. Wash off unbound antibodies and add anti-rabbit IgG-HRP conjugate. Wash the plates and add substrate to develop color for 5 min. Read absorbance at 405 nm. The intensity of the colour is directly proportional to the amount of antigen present.
    A positive is when the sample signal is double that of the control or pre-immune serum.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Taxol (paclitaxel) is particularly effective as an anticancer agent because tubulin is not its only target. It also binds to a protein called Bcl2, which normally blocks the process of apoptosis, or cell death. Taxol therefore plays a dual role in destroying dividing cells,firstly by stabilising assembled microtubules and thereby halting mitosis, and secondly by inhibiting Bcl2 and allowing apoptosis to proceed.
    • 别名
      • Paclitaxel antibody

    Anti-Taxol antibody (ab26953)参考文献

    ab26953 has not yet been referenced specifically in any publications.

    Product Wall

    Application ELISA
    Sample Rat Serum (oral)
    Specification oral
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
    Type Indirect
    Username

    Abcam user community

    Verified customer

    提交于 Dec 14 2015

    Thank you for contacting us.

    To our knowledge, the anti-Taxol ab26953 has not been tested in immunocytochemistry. However by participating in our AbTrial program you can now use our products in an untested application or species without financ...

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    Thank you for contacting us.

    The titre of this antibody as observed in indirect ELISA is more than 10,000.

    The below table show absorbance at 405nm using 0.4ug of antigen. HRP-conjugated anti rabbit ab was used.

    Ab= dilution ...

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    Thank you for your enquiry.

    I would appreciate if you could confirm you are referring toTaxol antibody product code ab26953?

    I am sorry to confirm that as far as we are aware, ab26953 has never been tested in ICC. All tested appli...

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    Thank you for your inquiry and your patience with this reply.
    I am sorry to report that the lab hasn't tested to verify the position of the conjugate on taxol. They do not have this information.
    I regret that this information has not been ver...

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    Thank you for contacting us.

    The link to our protocol for indirect ELISA is as follows:

    http://www.abcam.com/index.html?pageconfig=resource&rid=11389

    Another useful link to our practical guide for ELISA is:

    ...

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    Thank you for your enquiry. Unfortunately, we currently do not have any direct references regarding the use of this product. All of our references come from loyal customers like you, who have used our products, published their findings in journals a...

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    Thank you for your enquiry. Please rest assure that this product (ab26953) is an antibody, raised by immunizing rabbits with the Taxol conjugation tg. It is not the Taxol compound itself and would therefore not cause any health problems. This produ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"