Anti-Tau (phospho S356)抗体(ab75603)

概述

• 产品名称
  Anti-Tau (phospho S356)抗体
  参阅全部 Tau 一抗
• 描述
  兔多克隆抗体to Tau (phospho S356)
• 特异性
  ab75603 detects endogenous levels of Tau only when phosphorylated at serine 356.
• 经测试应用
  适用于: ICC/IF, WBmore details
• 种属反应性
  与反应: Mouse, Rat, Human
  
• 免疫原

  Synthetic phosphopeptide derived from human Tau around the phosphorylation site of serine 356 (I-G-S^P-L-D).

• 阳性对照
  • Extracts from rat brain tissue.

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
• 存储溶液
  Preservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• 纯度
  Immunogen affinity purified
• 纯化说明
  ab75603 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• 克隆
  多克隆
• 同种型
  IgG
• 研究领域
  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Tangles & Tau

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microtubules
  • MT Associated Proteins
  • Tau

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Axon marker

相关产品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Related Products
  • Human Tau ELISA Kit (ab210972)

应用

靶标

• 功能
  Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
• 组织特异性
  Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
• 疾病相关
  Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
  Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
  Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
  Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
  Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.
• 序列相似性
  Contains 4 Tau/MAP repeats.
• 发展阶段
  Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.
• 结构域
  The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
• 翻译后修饰
  Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
  Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
  Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
• 细胞定位
  Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.

• Target information above from: UniProt accession P10636 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 4137 Human
  • Entrez Gene: 17762 Mouse
  • Entrez Gene: 29477 Rat
  • Omim: 157140 Human
  • SwissProt: P10636 Human
  • SwissProt: P10637 Mouse
  • SwissProt: P19332 Rat
  • Unigene: 101174 Human

  • Unigene: 1287 Mouse
  • Unigene: 2455 Rat

  see all
• 形式
  There are 9 isoforms produced by alternative splicing.
• 别名
  • AI413597 antibody
  • AW045860 antibody
  • DDPAC antibody

  • FLJ31424 antibody
  • FTDP 17 antibody
  • G protein beta1/gamma2 subunit interacting factor 1 antibody
  • MAPT antibody
  • MAPTL antibody
  • MGC134287 antibody
  • MGC138549 antibody
  • MGC156663 antibody
  • Microtubule associated protein tau antibody
  • Microtubule associated protein tau isoform 4 antibody
  • Microtubule-associated protein tau antibody
  • MSTD antibody
  • Mtapt antibody
  • MTBT1 antibody
  • MTBT2 antibody
  • Neurofibrillary tangle protein antibody
  • Paired helical filament tau antibody
  • Paired helical filament-tau antibody
  • PHF tau antibody
  • PHF-tau antibody
  • PPND antibody
  • PPP1R103 antibody
  • Protein phosphatase 1, regulatory subunit 103 antibody
  • pTau antibody
  • RNPTAU antibody
  • TAU antibody
  • TAU_HUMAN antibody
  • Tauopathy and respiratory failure, included antibody

  see all

实验方案

• Western blot protocols
• Immunocytochemistry & immunofluorescence protocols