概述

  • 产品名称
    Anti-Tau 13抗体[B11E8]
  • 描述
    小鼠单克隆抗体[B11E8] to Tau 13
  • 特异性
    The antibody recognizes 45-60 kDa human Tau-13. Reactivity to other species has not been tested.
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Tau 13 (N terminal).
    Database link: P10636

  • 常规说明


    The antibody recognizes 45-60 kDa isoform of human Tau-13.

性能

应用

Our Abpromise guarantee covers the use of ab19030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 79 kDa.
IP 1/500.
IHC-P 1/100.

靶标

  • 相关性
    Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF)and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK 3 beta, PKA, Cdk 5, and casein kinase II can phosphorylate tau.
  • 细胞定位
    Cytoplasmic and Plasma membrane
  • 数据库链接
  • 别名
    • FTDP17 antibody
    • Microtubule associated protein tau antibody
    • Paired helical filament tau antibody
    • PHF tau antibody
    • PPND antibody
    • TAU antibody
    see all

图片

  • All lanes : Anti-Tau 13 antibody [B11E8] (ab19030) at 1/5000 dilution

    Lane 1 : iPSC-derived neuronal culture (Human) at 2.5 µg
    Lane 2 : iPSC-derived neuronal culture (Human) at 2.5 µg
    Lane 3 : iPSC-derived neuronal culture (Human) at 1 µg
    Lane 4 : Whole brain homogenate

    Secondary
    HRP conjugated Goat Anti-Mouse at 1/2500 dilution

    Predicted band size : 79 kDa

    This image is courtesy of an AbReview submitted by Martin Engel.

    See Abreview

文献

This product has been referenced in:
  • Lathuilière A  et al. Motifs in the tau protein that control binding to microtubules and aggregation determine pathological effects. Sci Rep 7:13556 (2017). ELISA ; Human . Read more (PubMed: 29051562) »

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (iPSC-derived neuronal culture)
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Loading amount
2.5 µg
Specification
iPSC-derived neuronal culture
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Martin Engel

Verified customer

提交于 Nov 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册