Anti-TATA binding protein TBP抗体(ab74222)

概述

• 产品名称
  Anti-TATA binding protein TBP抗体
  参阅全部 TATA binding protein TBP 一抗
• 描述
  兔多克隆抗体to TATA binding protein TBP
• 经测试应用
  适用于: WBmore details
  不适用于: IP
• 种属反应性
  与反应: Human
  预测可用于: Mouse, Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Snake, Rhesus monkey, Gorilla, Orangutan, Platypus
• 免疫原

  Synthetic peptide corresponding to a region between residues 1 and 50 of human TBP (NP_003185.1)

• 阳性对照
  • HeLa or 293T whole cell lysate

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• 存储溶液
  Preservative: 0.09% Sodium Azide
  Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
• Concentration information loading...
• 纯度
  Immunogen affinity purified
• 克隆
  多克隆
• 同种型
  IgG
• 研究领域
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TFIIB

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TAFs

  • Isotype/Loading Controls
  • Loading Controls
  • TBP - Nuclear

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Preinitiation complex

相关产品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Positive Controls
  • HeLa nuclear extract lysate (ab14655)
  • HeLa whole cell lysate (ab29545)

应用

靶标

• 功能
  General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
• 组织特异性
  Widely expressed, with levels highest in the testis and ovary.
• 疾病相关
  Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
• 序列相似性
  Belongs to the TBP family.
• 细胞定位
  Nucleus.

• Target information above from: UniProt accession P20226 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 395995 Chicken
  • Entrez Gene: 431689 Dog
  • Entrez Gene: 6908 Human
  • Entrez Gene: 21374 Mouse
  • Entrez Gene: 117526 Rat
  • Omim: 600075 Human
  • SwissProt: O13270 Chicken
  • SwissProt: P20226 Human

  • SwissProt: P29037 Mouse
  • Unigene: 1100 Human
  • Unigene: 590872 Human
  • Unigene: 244820 Mouse
  • Unigene: 22712 Rat

  see all
• 别名
  • GTF2D antibody
  • GTF2D1 antibody
  • HDL4 antibody

  • MGC117320 antibody
  • MGC126054 antibody
  • MGC126055 antibody
  • SCA17 antibody
  • TATA binding factor antibody
  • TATA box factor antibody
  • TATA sequence binding protein antibody
  • TATA sequence-binding protein antibody
  • TATA-binding factor antibody
  • TATA-box binding protein N-terminal domain antibody
  • TATA-box factor antibody
  • TATA-box-binding protein antibody
  • TBP antibody
  • TBP_HUMAN antibody
  • TFIID antibody
  • Transcription initiation factor TFIID TBP subunit antibody

  see all

实验方案

• Western blot protocols