Anti-TATA binding protein TBP抗体(ab24342)

概述

  • 产品名称Anti-TATA binding protein TBP抗体
    参阅全部 TATA binding protein TBP 一抗
  • 描述
    兔多克隆抗体to TATA binding protein TBP
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Cow, Dog, Caenorhabditis elegans, Chimpanzee, Zebrafish
  • 免疫原

    Synthetic peptide: GKMVCTGAKS EEQSRLAARK YARVVQKLGF PAKFLDFKIQ NMVGSCDVKF, corresponding to a region within internal sequence amino acids 217-266 of Human TATA binding protein TBP

  • 阳性对照
    • Fetal placenta cell lysate for WB.

性能

应用

Our Abpromise guarantee covers the use of ab24342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/62500.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
  • 组织特异性Widely expressed, with levels highest in the testis and ovary.
  • 疾病相关Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
  • 序列相似性Belongs to the TBP family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GTF2D antibody
    • GTF2D1 antibody
    • HDL4 antibody
    • MGC117320 antibody
    • MGC126054 antibody
    • MGC126055 antibody
    • SCA17 antibody
    • TATA binding factor antibody
    • TATA box factor antibody
    • TATA sequence binding protein antibody
    • TATA sequence-binding protein antibody
    • TATA-binding factor antibody
    • TATA-box binding protein N-terminal domain antibody
    • TATA-box factor antibody
    • TATA-box-binding protein antibody
    • TBP antibody
    • TBP_HUMAN antibody
    • TFIID antibody
    • Transcription initiation factor TFIID TBP subunit antibody
    see all

Anti-TATA binding protein TBP antibody 图像

  • Anti-TATA binding protein TBP antibody (ab24342) at 2.5 µg/ml + Fetal placenta cell lysate.

    Secondary
    HRP conjugated rabbit anti TBP antibody at a dilution of 1/50000 - 1/100000.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

Anti-TATA binding protein TBP antibody (ab24342)参考文献

ab24342 has not yet been referenced specifically in any publications.

Product Wall

Application ChIP
Detection step Real-time PCR
Sample Arabidopsis thaliana Tissue lysate - nuclear (chromatin prep)
Specification chromatin prep
Negative control serum control (ab6697)
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde (3%)
Positive control None The experimental setup (amount of chromatin solution) did not allow for a positive control
Username

Abcam user community

Verified customer

提交于 Jul 11 2014



For this antibody we loaded approx 20-25ug of total cell lysate/homogenate.

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Senecio chrysanthemifolius Cell lysate - nuclear (Leaf)
Specification Leaf
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step Real-time PCR
Positive control Input and Pi-tubulin
Negative control No-Ab
Username

Dr. Dhaval Patel

Verified customer

提交于 Feb 05 2013

I am very pleased to hear you would like to accept our offer and test ab24342 and ab817 in Sencio. These code will give you the value stated above off your next order before the expiration date. To redeem this offer, please submit an Abreview for Scenc...

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Thank you for your reply.

I can confirm the alignment of the TBP antibody ab24342 with the Senecio TBP sequence you provided is 88%. Therefore, I would suggest that ab24342 would be likely to detect the Senecio TBP protein.

The al...

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Thank you for your message.

I am pleased to review whether we are able to provide testing discounts for you.

In order to do so, as requested in my last email I would appreciate if you are able to provide the amino acid sequence fo...

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Thank you for your enquiry. I have requested that the lab performs an alignment of the immunogen sequences with the rat homologs. Details of the degree of similarity is below: BRD2(rat) - GeneID: 294276 (NP_997660) The peptide immunogen w...

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