Anti-Tartrate Resistant Acid Phosphatase抗体[26E5] (ab49507)

概述

  • 产品名称Anti-Tartrate Resistant Acid Phosphatase抗体[26E5]
    参阅全部 Tartrate Resistant Acid Phosphatase 一抗
  • 描述
    小鼠单克隆抗体[26E5] to Tartrate Resistant Acid Phosphatase
  • 特异性This antibody is specific for Tartrate Resistant Acid Phosphatase.
  • 经测试应用适用于: IHC-P, ICC/IFmore details
    不适用于: IHC-Fr or WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fusion protein corresponding to the N terminal portion of human Tartrate Resistant Acid Phosphatase (TRAP)

  • 阳性对照
    • Tonsil, Human osteoclastoma

性能

应用

Our Abpromise guarantee covers the use of ab49507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

We recommend high temperature antigen unmasking and 60 minutes primary antibody incubation at 25°C prior to commencing with IHC staining protocol.

ICC/IF 1/1000.
  • 应用说明Is unsuitable for IHC-Fr or WB.
  • 靶标

    • 功能Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
    • 序列相似性Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
    • 细胞定位Lysosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Type 5 acid phosphatase antibody
      • Acid phosphatase 5 tartrate resistant antibody
      • Acid phosphatase 5, tartrate resistant antibody
      • ACP5 antibody
      • EC 3.1.3.2 antibody
      • MGC117378 antibody
      • PPA5_HUMAN antibody
      • serum band 5 tartrate-resistant acid phosphatase antibody
      • SPENCDI antibody
      • T5ap antibody
      • Tartrate resistant acid ATPase antibody
      • Tartrate resistant acid phosphatase type 5 antibody
      • Tartrate resistant acid phosphatase type 5 precursor antibody
      • Tartrate-resistant acid ATPase antibody
      • Tartrate-resistant acid phosphatase type 5 antibody
      • TR AP antibody
      • TR-AP antibody
      • TRACP 5 antibody
      • TRAP antibody
      • TrATPase antibody
      • Type 5 acid phosphatase antibody
      see all

    Anti-Tartrate Resistant Acid Phosphatase antibody [26E5] 图像

    • ab49507 staining Tartate Resistance Acid Phosphatase in Human osteoclast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/100 in 0.1% Triton + 3% BSA) for 12 hours at 4°C. An undiluted Alexa Fluor® 555-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Immunohistochemical staining (Paraffin section) for Tartrate Resistant Acid Phosphatase using ab49507 with Human osteoclastoma as positive control. Note intense cytoplasmic staining of osteoclasts.
    • ICC/IF image of ab49507 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49507, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-Tartrate Resistant Acid Phosphatase antibody [26E5] (ab49507)参考文献

    This product has been referenced in:
    • Kawamura M  et al. Clinical significance of tartrate-resistant acid phosphatase type-5 expression in human gastric cancer. Anticancer Res 34:3425-9 (2014). IHC-P ; Human . Read more (PubMed: 24982350) »
    • Xia L  et al. ACP5, a direct transcriptional target of FoxM1, promotes tumor metastasis and indicates poor prognosis in hepatocellular carcinoma. Oncogene N/A:N/A (2013). Read more (PubMed: 23604121) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Sample Human Cell (osteoclast)
    Specification osteoclast
    Permeabilization Yes - 0.1% TritonX-100
    Fixative Paraformaldehyde
    Username

    Rekha Rao Manepalli

    Verified customer

    提交于 Sep 24 2013

    Oui, n'hésitez pas à me contacter de nouveau si vous avez plus d'informations concernant la différence entre la forme 5a et 5b, je serai ravi de chercher l'anticorps qui convient à vos travaux.

    Merci de nous avoir contactés.

    Les quatre anticorps anti-TRAP (type 5)que nous avons sont :
    ab49507
    ab96372
    ab75452
    ab14054
    Ils sont tous dirigés vers la protéine dont la référence UniProt...

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    Thank you for your reply. As I mentioned previously, we do not give free samples of our antibodies, even for testing purposes. We issue testing discount codes for antibodies that have not been tested in a specific application or species. How the testin...

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    Thank you for your reply. As mentioned in the previous emails, we do not send samples sizes of our antibodies, under our Abpomise we guarantees the antibodies to work as stated on the datasheet. If the antibodies do not work as stated then we will eith...

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    Thank you for your reply. I am sorry to confirm that as far as we are aware, ab49507 has not been tested with samples from mouse. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these are updated as s...

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    Thank you for your enquiry. I am pleased to confirm that ab49507 Tartrate Resistant Acid Phosphatase antibody has already been tested and is guaranteed in IHC-P. Therefore, I am sorry that a testing discount to test in IHC-P would not be appropriat...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"