Western blot - Tapasin Related Protein antibody (ab57411)
Predicted band size : 50 kDa Tapasin Related Protein antibody (ab57411) at 1ug/lane + A-431 cell lysate at 25ug/lane.
Flow Cytometry - Anti-Tapasin Related Protein antibody (ab57411)
Overlay histogram showing A431 cells stained with ab57411 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57411, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Anti-Tapasin Related Protein antibody (ab57411)参考文献
This product has been referenced in:
Boyle LH et al. Tapasin-related protein TAPBPR is an additional component of the MHC class I presentation pathway. Proc Natl Acad Sci U S A110:3465-70 (2013).
Read more (PubMed: 23401559) »