概述

  • 产品名称Anti-Tapasin抗体
    参阅全部 Tapasin 一抗
  • 描述
    兔多克隆抗体to Tapasin
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide:

    LGWAAVYLSTCKDSKKKAE

    , corresponding to amino acids 430-448 of Human Tapasin.

  • 阳性对照
    • This antibody gave a positive signal in A431 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab13518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/100.

靶标

  • 功能Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
  • 组织特异性Neutrophils, mostly in fully differentiated cells.
  • 序列相似性Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 结构域The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminus region.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NGS 17 antibody
    • NGS-17 antibody
    • NGS17 antibody
    • TAP associated protein antibody
    • TAP binding protein antibody
    • TAP binding protein (tapasin) antibody
    • TAP BP antibody
    • TAP-associated protein antibody
    • TAP-binding protein antibody
    • TAPA antibody
    • Tapasin antibody
    • TAPBP antibody
    • TPN antibody
    • TPSN antibody
    • TPSN_HUMAN antibody
    see all

Anti-Tapasin antibody 图像

  • Anti-Tapasin antibody (ab13518) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa


    Exposure time : 12 minutes

Anti-Tapasin antibody (ab13518)参考文献

This product has been referenced in:
  • Del Campo AB  et al. Adenovirus expressing ß2-microglobulin recovers HLA class I expression and antitumor immunity by increasing T-cell recognition. Cancer Gene Ther N/A:N/A (2014). Read more (PubMed: 24971583) »
  • Chapiro J  et al. Destructive cleavage of antigenic peptides either by the immunoproteasome or by the standard proteasome results in differential antigen presentation. J Immunol 176:1053-61 (2006). WB ; Human . Read more (PubMed: 16393993) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Fibroblast cell line)
Loading amount 100 µg
Specification Human Fibroblast cell line
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Dec 18 2012

I'm sorry to hear your customer is having problems with ab13518 and thank him for taking the time to give us protocol details. We have not received any complaints regarding this antibody and are confused by the complaint form: the customer mentions...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"