Anti-TAPA1抗体[M38] (Phycoerythrin) (ab81436)

概述

  • 产品名称Anti-TAPA1抗体[M38] (Phycoerythrin)
    参阅全部 TAPA1 一抗
  • 描述
    小鼠单克隆抗体[M38] to TAPA1 (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用适用于: WB, IP, ICC, IHC-P, Functional Studies, Flow Cytmore details
  • 种属反应性
    与反应: Rabbit, Cat, Human
  • 免疫原

    MOLT-4 (Human T-ALL cell line)

  • 常规说明The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.


    In human MOLT-4 T-cell line the ab81436 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.

性能

应用

Our Abpromise guarantee covers the use of ab81436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 106 cells in a suspension.



ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.
  • 组织特异性Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.
  • 疾病相关Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • 序列相似性Belongs to the tetraspanin (TM4SF) family.
  • 翻译后修饰Not glycosylated.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 26 kDa cell surface protein TAPA 1 antibody
    • 26 kDa cell surface protein TAPA-1 antibody
    • 26 kDa cell surface protein TAPA1 antibody
    • CD 81 antibody
    • CD81 antibody
    • CD81 antigen (target of antiproliferative antibody 1) antibody
    • CD81 antigen antibody
    • CD81 molecule antibody
    • CD81_HUMAN antibody
    • CVID6 antibody
    • S5.7 antibody
    • TAPA 1 antibody
    • TAPA1 antibody
    • Target of the antiproliferative antibody 1 antibody
    • Tetraspanin 28 antibody
    • Tetraspanin-28 antibody
    • Tetraspanin28 antibody
    • Tspan 28 antibody
    • Tspan-28 antibody
    • Tspan28 antibody
    see all

Anti-TAPA1 antibody [M38] (Phycoerythrin) (ab81436)参考文献

This product has been referenced in:
  • Escola JM  et al. Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem 273:20121-7 (1998). Read more (PubMed: 9685355) »
  • Imai T & Yoshie O C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol 151:6470-81 (1993). IP ; Human . Read more (PubMed: 8245480) »

See all 3 Publications for this product

Product Wall

Thank you for your reply. I appreciate the time taken to submit protocol information and I would like to make some suggestions to the protocol in an attempt to improve the results obtained. -I would recommend fixation for shorter time. Fixing in par...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I a...

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Thank you for contacting us. If you are not able to easily pellet the exosomes, I would indeed recommend to add 20 µl of the antibody solution to the exosomes in 100 µl PBS. You may need to increase the incubation time and or the volume of ab81436 a...

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Thank you for contacting us. I can confirm that the PE-conjugated CD81 antibody ab81436 is designed for Flow Cytometry analysis of human blood cells, and if the volume of your whole blood sample is 100 µl as indicated, we would suggest the following...

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Thank you for your inquiry. Unfortunately, we do not have information about the concentration of ab81436. Antibody concentration is usually determined by measuring the protein concentration. This is not useful in this case since PE is a...

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