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Tissue, cells or virus corresponding to Human TAPA1. Aggregated OCI-LY8 cells.
Ab35026 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti proliferative effects. Endotoxin Level: <0.01 Eu/ug (low endotoxin grade).
Our Abpromise guarantee covers the use of ab35026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
|IHC-P||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 26 kDa.
|Functional Studies||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
ab35026 staining TAPA1 in SH-SY5Y were transfected with cDNA encoding CD81 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 3% BSA for 2 hours at room temperature. Samples were incubated with primary antibody (1/100 in PBS + 3% BSA) for 2 hours. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.