Anti-TAPA1抗体[1D6] (ab35026)

概述

  • 产品名称Anti-TAPA1抗体[1D6]
    参阅全部 TAPA1 一抗
  • 描述
    小鼠单克隆抗体[1D6] to TAPA1
  • 特异性ab35026 recognises CD81, a 26kD cell surface antigen and a member of the tetraspanin family.
  • 经测试应用适用于: Flow Cyt, IHC-P, IP, WB, Functional Studies, ICC/IFmore details
  • 种属反应性
    与反应: Human, Chimpanzee
  • 免疫原

    Tissue, cells or virus corresponding to Human TAPA1. Aggregated OCI-LY8 cells.

  • 常规说明


    Ab35026 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti proliferative effects. Endotoxin Level: <0.01 Eu/ug (low endotoxin grade).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明This antibody was purified from tissue culture supernatant.
  • Primary antibody说明Ab35026 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti proliferative effects. Endotoxin Level: <0.01 Eu/ug (low endotoxin grade).
  • 克隆单克隆
  • 克隆编号1D6
  • 骨髓瘤P3x63-Ag8.653
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab35026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/10.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 26 kDa.

PMID:20224111

Functional Studies Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.
  • 组织特异性Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.
  • 疾病相关Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • 序列相似性Belongs to the tetraspanin (TM4SF) family.
  • 翻译后修饰Not glycosylated.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 26 kDa cell surface protein TAPA 1 antibody
    • 26 kDa cell surface protein TAPA-1 antibody
    • 26 kDa cell surface protein TAPA1 antibody
    • CD 81 antibody
    • CD81 antibody
    • CD81 antigen (target of antiproliferative antibody 1) antibody
    • CD81 antigen antibody
    • CD81 molecule antibody
    • CD81_HUMAN antibody
    • CVID6 antibody
    • S5.7 antibody
    • TAPA 1 antibody
    • TAPA1 antibody
    • Target of the antiproliferative antibody 1 antibody
    • Tetraspanin 28 antibody
    • Tetraspanin-28 antibody
    • Tetraspanin28 antibody
    • Tspan 28 antibody
    • Tspan-28 antibody
    • Tspan28 antibody
    see all

Anti-TAPA1 antibody [1D6] 图像

  • ab35026 staining TAPA1 in SH-SY5Y were transfected with cDNA encoding CD81 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 3% BSA for 2 hours at room temperature. Samples were incubated with primary antibody (1/100 in PBS + 3% BSA) for 2 hours. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Flow cytometry analysis of Human peripheral blood lymphocytes, staining TAPA1 with ab35026 at 1/10 dilution.

Anti-TAPA1 antibody [1D6] (ab35026)参考文献

This product has been referenced in:
  • Drummond HA  et al. Renal inflammation and elevated blood pressure in a mouse model of reduced {beta}-ENaC. Am J Physiol Renal Physiol 301:F443-9 (2011). WB ; Mouse . Read more (PubMed: 21543417) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (SH-SY5Y were transfected with cDNA encoding CD81)
Permeabilization Yes - 0,2% Triton PBS
Specification SH-SY5Y were transfected with cDNA encoding CD81
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
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Verified customer

提交于 Jul 29 2015

Thank you for contacting us today in regards to the Anti-TAPA1 antibody [1D6] - Azide free (ab35026).

As discussed over the phone, I have checked into whether the antibody has been used in ICC. I can confirm that the antibody has been used in ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"