概述

  • 产品名称Anti-TAP1抗体
    参阅全部 TAP1 一抗
  • 描述
    兔多克隆抗体to TAP1
  • 特异性No reaction was observed against TAP-II in ELISA or immunoblotting.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Transporter Associated with Antigen Processing (TAP1 ) peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

  • 常规说明


    Transporter Associated with Antigen Processing (TAP) is involved in the delivery of peptides generated from proteolytic degradation of cytoplasmic antigens across the ER to class I MHC.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis.
  • Primary antibody说明Transporter Associated with Antigen Processing (TAP) is involved in the delivery of peptides generated from proteolytic degradation of cytoplasmic antigens across the ER to class I MHC.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
  • 疾病相关Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1) [MIM:604571]; also called HLA class I deficiency. BLS1 is a class I antigen deficiency that is not accompanied by particular pathologic manifestations during the first years of life. Systemic infections have not been described. Chronic bacterial infections, often beginning in the first decade of life, are restricted to the respiratory tract.
  • 序列相似性Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 1 ABC transporter domain.
  • 结构域The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.
  • 细胞定位Endoplasmic reticulum membrane. The transmembrane segments seem to form a pore in the membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABC 17 antibody
    • ABC transporter MHC 1 antibody
    • ABC17 antibody
    • ABCB 2 antibody
    • ABCB2 antibody
    • Antigen peptide transporter 1 antibody
    • APT 1 antibody
    • APT1 antibody
    • ATP binding cassette sub family B (MDR/TAP) member 2 antibody
    • ATP binding cassette sub family B member 2 antibody
    • ATP binding cassette transporter antibody
    • ATP-binding cassette sub-family B member 2 antibody
    • D6S114E antibody
    • FLJ26666 antibody
    • FLJ41500 antibody
    • Peptide supply factor 1 antibody
    • Peptide transporter involved in antigen processing 1 antibody
    • Peptide transporter PSF 1 antibody
    • Peptide transporter PSF1 antibody
    • Peptide transporter TAP 1 antibody
    • Peptide transporter TAP1 antibody
    • PSF 1 antibody
    • PSF-1 antibody
    • PSF1 antibody
    • Really interesting new gene 4 protein antibody
    • RING 4 antibody
    • RING4 antibody
    • TAP 1 antibody
    • TAP1 antibody
    • TAP1*0102N antibody
    • TAP1_HUMAN antibody
    • TAP1N antibody
    • Transporter 1 ATP binding cassette sub family B (MDR/TAP) antibody
    • Transporter 1 ATP binding cassette sub family B antibody
    • Transporter associated with antigen processing antibody
    • Transporter ATP binding cassette major histocompatibility complex 1 antibody
    • Y3 antibody
    see all

Anti-TAP1 antibody (ab2099)参考文献

This product has been referenced in:
  • Spadaro F  et al. IFN-a enhances cross-presentation in human dendritic cells by modulating antigen survival, endocytic routing, and processing. Blood 119:1407-17 (2012). ICC/IF ; Human . Read more (PubMed: 22184405) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (melanoma)
Loading amount 50 µg
Specification melanoma
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Jun 08 2007

Please see our FACS protocol. A link is present on the right hand side of the ABCAM homepage.

Reactivity with other mammalian sources is unknown, but likely, due to the nature of the polyclonal antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"