概述

  • 产品名称Anti-TAP1抗体
    参阅全部 TAP1 一抗
  • 描述
    兔多克隆抗体to TAP1
  • 特异性This antibody detects an ~70 kDa protein, corresponding to the apparent molecular mass of Transporter Associated with Antigen Processing 1 (TAP1) on SDS-PAGE immunoblots. The immunogen sequence used to generate this antibody is based on the sequence of ATP-binding cassette sub-family B member 2.
  • 经测试应用适用于: WB, IP, ICCmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide:

    CYWAMVQAPADAPE

    , corresponding to amino acids 735-748 of Human TAP1 (member 2).

  • 阳性对照
    • HeLa cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: Whole Serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab13516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/4000. Detects a band of approximately 70 kDa.
IP 1/100.
ICC Use at an assay dependent dilution.

靶标

  • 功能Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
  • 疾病相关Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1) [MIM:604571]; also called HLA class I deficiency. BLS1 is a class I antigen deficiency that is not accompanied by particular pathologic manifestations during the first years of life. Systemic infections have not been described. Chronic bacterial infections, often beginning in the first decade of life, are restricted to the respiratory tract.
  • 序列相似性Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 1 ABC transporter domain.
  • 结构域The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.
  • 细胞定位Endoplasmic reticulum membrane. The transmembrane segments seem to form a pore in the membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABC 17 antibody
    • ABC transporter MHC 1 antibody
    • ABC17 antibody
    • ABCB 2 antibody
    • ABCB2 antibody
    • Antigen peptide transporter 1 antibody
    • APT 1 antibody
    • APT1 antibody
    • ATP binding cassette sub family B (MDR/TAP) member 2 antibody
    • ATP binding cassette sub family B member 2 antibody
    • ATP binding cassette transporter antibody
    • ATP-binding cassette sub-family B member 2 antibody
    • D6S114E antibody
    • FLJ26666 antibody
    • FLJ41500 antibody
    • Peptide supply factor 1 antibody
    • Peptide transporter involved in antigen processing 1 antibody
    • Peptide transporter PSF 1 antibody
    • Peptide transporter PSF1 antibody
    • Peptide transporter TAP 1 antibody
    • Peptide transporter TAP1 antibody
    • PSF 1 antibody
    • PSF-1 antibody
    • PSF1 antibody
    • Really interesting new gene 4 protein antibody
    • RING 4 antibody
    • RING4 antibody
    • TAP 1 antibody
    • TAP1 antibody
    • TAP1*0102N antibody
    • TAP1_HUMAN antibody
    • TAP1N antibody
    • Transporter 1 ATP binding cassette sub family B (MDR/TAP) antibody
    • Transporter 1 ATP binding cassette sub family B antibody
    • Transporter associated with antigen processing antibody
    • Transporter ATP binding cassette major histocompatibility complex 1 antibody
    • Y3 antibody
    see all

Anti-TAP1 antibody (ab13516)参考文献

This product has been referenced in:
  • Zhang SY  et al. HMME-based PDT restores expression and function of transporter associated with antigen processing 1 (TAP1) and surface presentation of MHC class I antigen in human glioma. J Neurooncol 105:199-210 (2011). WB . Read more (PubMed: 21520005) »
  • Larrea E  et al. Oncostatin M enhances the antiviral effects of type I interferon and activates immunostimulatory functions in liver epithelial cells. J Virol 83:3298-311 (2009). WB ; Human . Read more (PubMed: 19158240) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Fibroblast cell line)
Loading amount 100 µg
Specification Human Fibroblast cell line
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Dec 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"