TAOK3 overexpression 293T裂解液(全细胞) (ab94147)

概述

  • 产品名称TAOK3 overexpression 293T裂解液(全细胞)
  • 描述
    TAOK3 overexpression裂解液
  • 常规说明ab94147 is a 293T cell transfected lysate in which Human JIK has been transiently over-expressed using a pCMV-JIK. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK. Tissue specificity: Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain. PTM: Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.

应用

Our Abpromise guarantee covers the use of ab94147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

TAOK3 overexpression 293T lysate (whole cell) 图像

  • ab94147 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-TAOK3 antibody (ab57359) at 1/500 dilution

    Lane 1 : TAOK3 overexpression 293T lysate (whole cell) (ab94147)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

TAOK3 overexpression 293T lysate (whole cell) (ab94147)参考文献

ab94147 has not yet been referenced specifically in any publications.

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