• 产品名称Anti-TAOK3抗体
    参阅全部 TAOK3 一抗
  • 描述
    兔多克隆抗体to TAOK3
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to Human TAOK3 (C terminal).
    Database link: Q9H2K8

  • 阳性对照
    • HeLa whole cell lysates.



Our Abpromise guarantee covers the use of ab70297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明IP: Use at 1-5µg/mg of lysate.
    WB: 1/5000 - 1/25000. Detects a band of approximately 120 kDa (predicted molecular weight: 105 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK.
    • 组织特异性Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver.
    • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Cytoplasm. Cell membrane. Also localized to the peripheral cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CTCL tumor antigen HD CL 09 antibody
      • CTCL-associated antigen HD-CL-09 antibody
      • Cutaneous T cell lymphoma tumor antigen HD CL 09 antibody
      • Cutaneous T-cell lymphoma-associated antigen HD-CL-09 antibody
      • Dendritic cell derived protein kinase antibody
      • Dendritic cell-derived protein kinase antibody
      • DKFZp666H245 antibody
      • DPK antibody
      • FLJ31808 antibody
      • hKFC A antibody
      • hKFC-A antibody
      • JIK antibody
      • JNK/SAPK inhibitory kinase antibody
      • JNK/SAPK-inhibitory kinase antibody
      • Jun kinase inhibitory kinase antibody
      • Jun kinase-inhibitory kinase antibody
      • KDS antibody
      • Kinase from chicken homolog A antibody
      • MAP3K18 antibody
      • Serine kinase antibody
      • Serine/threonine-protein kinase TAO3 antibody
      • STE20 like kinase antibody
      • TAO kinase 3 antibody
      • TAOK3 antibody
      • TAOK3_HUMAN antibody
      • Thousand and one amino acid protein 3 antibody
      see all

    Anti-TAOK3 antibody 图像

    • All lanes : Anti-TAOK3 antibody (ab70297) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg

      Developed using the ECL technique

      Predicted band size : 105 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • Western Blot showing Lane 2: ab70297 staining JIK in HeLa whole cell lysates immunoprecipitated using ab70297 at 3µg/mg lysate (1mg/lane). Lane 1: control IgG used for immunoprecipitation.Detection: Chemiluminescence with an exposure time of 3 minutes

    Anti-TAOK3 antibody (ab70297)参考文献

    ab70297 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Murine embryonic fibroblast)
    Gel Running Conditions Non-reduced Denaturing (gel 10%)
    Loading amount 20 µg
    Treatment siRNA trasnfection directed against TAOK3
    Specification Murine embryonic fibroblast
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Bastiaan Maes

    Verified customer

    提交于 May 02 2016

    Thank you for taking the time to complete our questionnaire as requested.

    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.


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    Thank you for taking the time to contact us and for your telephone call yesterday afternoon. I appreciate your sending a message, I did in factunfortunately have your email address incorrectand am pleased to have confirmation of this.

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    Application Western blot
    Sample Mouse Tissue lysate - whole (Spleen)
    Loading amount 100 µg
    Specification Spleen
    Gel Running Conditions Reduced Denaturing (6% running section)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    提交于 May 18 2009