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RabMAb

Anti-Talin 2抗体[EPR2536(2)] (ab108967)

概述

  • 产品名称
    Anti-Talin 2抗体[EPR2536(2)]
    参阅全部 Talin 2 一抗
  • 描述
    兔单克隆抗体[EPR2536(2)] to Talin 2
  • 经测试应用
    适用于: WBmore details
    不适用于: ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Talin 2 aa 400-500.

  • 阳性对照
    • COS-1, 293T, Hela, and Jurkat cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab108967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 270 kDa (predicted molecular weight: 272 kDa).
  • 应用说明
    Is unsuitable for ICC/IF or IHC-P.
  • 靶标

    图片

    • All lanes : Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (purified)

      Lane 1 : Mouse heart
      Lane 2 : Rat heart

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 272 kDa
      Observed band size : 270 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (purified) + Jurkat cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 272 kDa
      Observed band size : 270 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (purified) + COS-1 cell lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 272 kDa
      Observed band size : 270 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (unpurified)

      Lane 1 : COS-1 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 272 kDa
      Observed band size : 270 kDa (why is the actual band size different from the predicted?)

    实验方案

    文献

    This product has been referenced in:

    See all 2 Publications for this product

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab108967.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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