概述

  • 产品名称Anti-Talin 1抗体
    参阅全部 Talin 1 一抗
  • 描述
    兔多克隆抗体to Talin 1
  • 经测试应用适用于: ICC/IF, IHC-P, WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1650 - 1750 of Human Talin 1.

    (Peptide available as ab71332.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat, Ramos, MEF1

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 270 kDa (predicted molecular weight: 270 kDa).Can be blocked with Human Talin 1 peptide (ab71332).
Flow Cyt Use a concentration of 3 - 4 µg/ml. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

Anti-Talin 1 antibody 图像

  • IHC image of Talin 1 staining in Human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71333, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-Talin 1 antibody (ab71333) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 270 kDa
    Observed band size : 270 kDa
    Additional bands at : 100 kDa,230 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

    Sample: mouse endoderm cell

    Preparation:

    Fix in 3% PFA in PBS for 30 min at RT

    Primary antibody: Rabbit anti-talin (ab71333), 1:100

    Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3® 488) preadsorbed (ab97075), 1:100

    Nuclear counterstain: DAPI

    Rhodamine-phalloidin, 1:100

    Nuclei were counterstained with DAPI

  • ICC/IF image of ab71333 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71333, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • FlowCyt data showing mouse platelet cells stained for Talin 1 using ab71333 (3.3µg/ml). The cells were fixed in paraformaldehyde and permeabilized with 0.1% soponin. The cells were then incubated with primary antibody (3.3µg/ml) for 20 mins. The cells were then incubated with Goat polyclonal to anti-rabbit IgG conjugated to Dylight-649.

    See Abreview

  • All lanes : Anti-Talin 1 antibody (ab71333) at 1/1000 dilution

    Lane 1 : Human platelets
    Lane 2 : Mouse platelets

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to rabbit IgG conjugated to IRDye 800CW (undiluted)

    Performed under reducing conditions.

    Predicted band size : 270 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous abreview.

    Detection method: Licor System

    See Abreview

Anti-Talin 1 antibody (ab71333)参考文献

This product has been referenced in:
  • Wang SL  et al. Osteopontin (OPN) is an important protein to mediate improvements in the biocompatibility of C ion-implanted silicone rubber. PLoS One 9:e98320 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24911051) »
  • De Semir D  et al. Pleckstrin homology domain-interacting protein (PHIP) as a marker and mediator of melanoma metastasis. Proc Natl Acad Sci U S A 109:7067-72 (2012). ICC/IF . Read more (PubMed: 22511720) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - other (Platelet)
Specification Platelet
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Khon Huynh

Verified customer

提交于 Jul 17 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (platelet)
Loading amount 20 µg
Specification platelet
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Nov 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (platelet)
Specification platelet
Preparation Cell harvesting/tissue preparation method: fixed, washed platelets from whole blood
Sample buffer: walsh buffer
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% saponin
Gating Strategy settings for platelet population, ungated
Username

Abcam user community

Verified customer

提交于 Sep 21 2012

Thanks you for confirming the details.

The protocol looks fine to me so I am sorry we are unable to determine why the antibody not able to detect right size band? Perhaps you can try following steps for troubleshooting
- 70-80% cells confl...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

-...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"