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RabMAb

Anti-TAK1 (phospho S439)抗体[EPR2863] (ab109404)

概述

  • 产品名称
    Anti-TAK1 (phospho S439)抗体[EPR2863]
    参阅全部 TAK1 一抗
  • 描述
    兔单克隆抗体[EPR2863] to TAK1 (phospho S439)
  • 特异性
    ab109404 only detects TAK1 phosphorylated at Serine 439 in Human or TAK1 phosphorylated at Serine 412 in Mouse.
  • 经测试应用
    适用于: WB, IP, ICCmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Phospho specific peptide against residues surrounding Serine 439 of Human TAK1.

  • 阳性对照
    • HeLa cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

应用

Our Abpromise guarantee covers the use of ab109404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa).
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
  • 应用说明
    Is unsuitable for Flow Cyt or IHC-P.
  • 靶标

    • 功能
      Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.
    • 序列相似性
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰
      Association with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
      Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP.
    • Information by UniProt
    • 数据库链接
    • 别名
      • M3K7_HUMAN antibody
      • MAP3K 7 antibody
      • Map3k7 antibody
      • MEKK7 antibody
      • Mitogen activated protein kinase kinase kinase 7 antibody
      • Mitogen-activated protein kinase kinase kinase 7 antibody
      • TAK1 antibody
      • TGF beta activated kinase 1 antibody
      • TGF-beta-activated kinase 1 antibody
      • TGF1a antibody
      • Transforming growth factor beta activated kinase 1 antibody
      • Transforming growth factor-beta-activated kinase 1 antibody
      see all

    图片

    • All lanes : Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1ß for 10 minutes whole cell lysate
      Lane 3 : HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1ß for 10 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 67 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 seconds

      Dilution and blocking buffer: 5% NFDM/TBST.

    • Dot Blot analysis of Lane 1: TAK1 (phospho S439) phospho peptide and Lane 2: TAK1 non-phospho peptide labeling TAK1 (phospho S439) with ab109404 at 1/1000 dilution (0.009 μg/ml). 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100,000 dilution. Exposure time: 3 minutes.

    • All lanes : Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with transforming growth factor-beta (TGF-beta)

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 67 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

    文献

    This product has been referenced in:
    • Liu Z  et al. Activation of TGF-ß activated kinase 1 promotes colon mucosal pathogenesis in inflammatory bowel disease. Physiol Rep 5:N/A (2017). Read more (PubMed: 28373409) »
    • Zhang D  et al. TGFß-activated Kinase 1 (TAK1) Inhibition by 5Z-7-Oxozeaenol Attenuates Early Brain Injury after Experimental Subarachnoid Hemorrhage. J Biol Chem 290:19900-9 (2015). Read more (PubMed: 26100626) »

    See all 2 Publications for this product

    客户评价及客户问答

    Thank you for contacting us and your interest in our products.

    Unfortunately, I can say that the anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) has been tried in IHC of paraffin embedded tissue sections, however it was found not to be...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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