Anti-TAGLN2抗体(ab121146)
Key features and details
- Rabbit polyclonal to TAGLN2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-TAGLN2抗体 -
描述
兔多克隆抗体to TAGLN2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
antigen, corresponding to the N-terminal region amino acids 4-136 of the Human TAGLN2 protein (P37802).
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阳性对照
- IHC-P: Human bone marrow tissue. WB: NIH/3T3, NBT-II, A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121146于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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说明 |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
靶标
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序列相似性
Belongs to the calponin family.
Contains 1 calponin-like repeat.
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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数据库链接
- Entrez Gene: 8407 Human
- Entrez Gene: 21346 Mouse
- Entrez Gene: 304983 Rat
- Omim: 604634 Human
- SwissProt: P37802 Human
- SwissProt: Q9WVA4 Mouse
- SwissProt: Q5XFX0 Rat
- Unigene: 517168 Human
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别名
- CDABP0035 antibody
- epididymis tissue protein Li 7e antibody
- HA1756 antibody
see all
图片
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (actin filaments). Recommended concentration of ab121146 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Lane 2 : Anti-TAGLN2 antibody (ab121146) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : A431 cells -
Immunohistochemical staining of Human bone marrow with ab121146 at 1/500 dilution.
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All lanes : Anti-TAGLN2 antibody (ab121146) at 0.4 µg/ml
Lane 1 : NIH/3T3 cells
Lane 2 : NBT-II cells
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab121146 被引用在 7 文献中.
- Wang H et al. TAGLN2-Regulated Trophoblast Migration, Invasion and Fusion are Impaired in Preeclampsia. Front Cell Dev Biol 10:810633 (2022). PubMed: 35281112
- Saito T et al. Determining the domain-level reaction-diffusion properties of an actin-binding protein transgelin-2 within cells. Exp Cell Res 404:112619 (2021). PubMed: 33965400
- Li Z et al. MicroRNA-133b alleviates doxorubicin-induced cardiomyocyte apoptosis and cardiac fibrosis by targeting PTBP1 and TAGLN2. Int J Mol Med 48:N/A (2021). PubMed: 33982775
- Sharma A et al. Transgelin-2 and phosphoregulation of the LIC2 subunit of dynein govern mitotic spindle orientation. J Cell Sci 133:N/A (2020). PubMed: 32467330
- Zhao F et al. MicroRNA-133b suppresses bladder cancer malignancy by targeting TAGLN2-mediated cell cycle. J Cell Physiol 234:4910-4923 (2019). PubMed: 30317571
- Zhao F et al. Identification of hub miRNA biomarkers for bladder cancer by weighted gene coexpression network analysis. Onco Targets Ther 10:5551-5559 (2017). PubMed: 29200870
- Xiao G et al. MiR-133b Regulates the Expression of the Actin Protein TAGLN2 during Oocyte Growth and Maturation: A Potential Target for Infertility Therapy. PLoS One 9:e100751 (2014). WB . PubMed: 24959893