重组Anti-TAG1/TAX1抗体[EPR5106] (ab133498)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5106] to TAG1/TAX1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TAG1/TAX1抗体[EPR5106]
参阅全部 TAG1/TAX1 一抗 -
描述
兔单克隆抗体[EPR5106] to TAG1/TAX1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide within Human TAG1/TAX1 aa 1-100. The exact sequence is proprietary.
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阳性对照
- Fetal brain, Human cerebellum, U87-MG, and SH-SY5Y cell lysates, U87-MG cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 3.02 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5106 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133498于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Detects a band of approximately 135 kDa (predicted molecular weight: 113 kDa).
|
说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 135 kDa (predicted molecular weight: 113 kDa). |
靶标
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功能
May play a role in the initial growth and guidance of axons. May be involved in cell adhesion. -
序列相似性
Belongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
细胞定位
Cell membrane. Attached to the neuronal membrane by a GPI-anchor and is also released from neurons. - Information by UniProt
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数据库链接
- Entrez Gene: 6900 Human
- Entrez Gene: 21367 Mouse
- Omim: 190197 Human
- SwissProt: Q02246 Human
- SwissProt: Q61330 Mouse
- Unigene: 519220 Human
- Unigene: 260861 Mouse
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别名
- Axonal glycoprotein TAG 1 antibody
- Axonal glycoprotein TAG-1 antibody
- Axonal glycoprotein TAG1 antibody
see all
图片
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All lanes : Anti-TAG1/TAX1 antibody [EPR5106] (ab133498) at 1/10000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : Human cerebellum lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 113 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometrical analysis of permeablized U87-MG cells using ab133498 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab133498 被引用在 3 文献中.
- Kuwayama R et al. Establishment of mouse model of inherited PIGO deficiency and therapeutic potential of AAV-based gene therapy. Nat Commun 13:3107 (2022). PubMed: 35661110
- Fathi A et al. Discovery of Novel Cell Surface Markers for Purification of Embryonic Dopamine Progenitors for Transplantation in Parkinson's Disease Animal Models. Mol Cell Proteomics 17:1670-1684 (2018). PubMed: 29848781
- Zhen YB et al. Expression of TAG1/APP signaling pathway in the proliferation and differentiation of glioma stem cells. Oncol Lett 14:2137-2140 (2017). PubMed: 28789439