概述

  • 产品名称Anti-TAF8抗体
    参阅全部 TAF8 一抗
  • 描述
    小鼠多克隆抗体to TAF8
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human TAF8 protein (AAH33728.1, or Q7Z7C8-3)

  • 阳性对照
    • TAF8 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab77059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 19 kDa for isoform 3 and 34 kDa for isoform 1.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo.
    • 序列相似性Belongs to the TAF8 family.
      Contains 1 histone-fold domain.
    • 结构域Ectopic expression of the histone fold domain acts as a dominant-negative mutant resulting in differentiation inhibition.
    • 细胞定位Nucleus. Cytoplasm. Localized in the cytoplasm and transported from the cytoplasm to the nucleus in some cells, possibly depending on the functional or developmental state of the cell.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ32821 antibody
      • hTAFII43 antibody
      • Protein taube nuss antibody
      • TAF(II)43 antibody
      • taf8 antibody
      • TAF8 RNA polymerase II, TATA box binding protein (TBP) associated factor, 43kDa antibody
      • TAF8_HUMAN antibody
      • TAFII-43 antibody
      • TAFII43 antibody
      • TATA box binding protein (TBP) associated factor, RNA polymerase II A 45/50kDa antibody
      • Taube nuss homolog (mouse) antibody
      • TBN antibody
      • TBP associated factor 8 antibody
      • TBP associated factor TAFII43 antibody
      • TBP associated factor, RNA polymerase II, 43 kD antibody
      • TBP-associated factor 43 kDa antibody
      • TBP-associated factor 8 antibody
      • Transcription initiation factor TFIID 43 kDa subunit antibody
      • Transcription initiation factor TFIID subunit 8 antibody
      see all

    Anti-TAF8 antibody 图像

    • All lanes : Anti-TAF8 antibody (ab77059) at 5 µg/ml

      Lane 1 : TAF8 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 19 kDa
      Observed band size : 19 kDa

    Anti-TAF8 antibody (ab77059)参考文献

    ab77059 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK 293T)
    Gel Running Conditions Reduced Denaturing (15%)
    Loading amount 50 µg
    Specification HEK 293T
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

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    提交于 Sep 22 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
    Sample Human Tissue sections (Human fibromatosis)
    Specification Human fibromatosis
    Permeabilization Yes - Tween-20
    Fixative Formaldehyde
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    提交于 Mar 13 2015

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 1°C
    Sample Mouse Cell (MSC)
    Specification MSC
    Permeabilization Yes - Triton 1%
    Fixative Formaldehyde
    Username

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    Verified customer

    提交于 Mar 04 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"