概述

  • 产品名称Anti-TAF7抗体
    参阅全部 TAF7 一抗
  • 描述
    小鼠单克隆抗体to TAF7
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: FIWNHGITLP LKNVRKRRFR KTAKKKYIES PDVEKEVKRL LSTDAEAVST RWEIIAEDET KEAENQGLDI SSPGMSGHRQ GHDSLEHDEL REIFN, corresponding to amino acids 130-224 of Human TAF7

性能

应用

Our Abpromise guarantee covers the use of ab57494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa.
IHC-P Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively).
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the TAF7 family.
  • 结构域The [KR]-[STA]-K motif is specifically recognized by the SETD7 methyltransferase, which methylates Lys-5 in vitro.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • RNA polymerase II TBP-associated factor subunit F antibody
    • TAF 7 antibody
    • TAF(II)55 antibody
    • TAF2F antibody
    • TAF7 antibody
    • TAF7 RNA polymerase II antibody
    • TAF7 RNA polymerase II TATA box binding protein (TBP) associated factor 55 kDa antibody
    • TAF7_HUMAN antibody
    • TAFII 55 antibody
    • TAFII-55 antibody
    • TAFII55 antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II F 55kD antibody
    • TATA box binding protein associated factor 2F antibody
    • TBP associated factor F antibody
    • Transcription factor IID subunit TAFII55 antibody
    • Transcription initiation factor TFIID 55 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 7 antibody
    see all

Anti-TAF7 antibody 图像

  • Overlay histogram showing HeLa cells stained with ab57494 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57494, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • TAF7 antibody (ab57494) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human lymph node.


  • Predicted band size : 40 kDa
    TAF7 antibody (ab57494) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
  • Lane 2 : Anti-TAF7 antibody (ab57494) at 1/1250 dilution
    Lane 3 : Anti-TAF7 antibody (ab57494) at 1/2500 dilution
    Lane 4 : Anti-TAF7 antibody (ab57494) at 1/5000 dilution
    Lane 5 : Anti-TAF7 antibody (ab57494) at 1/10000 dilution

    Lane 2 : whole cell lysate prepared from SW780 badder cancer cells
    Lane 3 : whole cell lysate prepared from SW780 badder cancer cells
    Lane 4 : whole cell lysate prepared from SW780 badder cancer cells
    Lane 5 : whole cell lysate prepared from SW780 badder cancer cells

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG conjugated to HRP
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

    Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview

    Lane 1: All Blue Biorad.
    Gel run under denaturing conditions with a 4-12% gradient.
    Blocking step performed using 5% milk for one hour at room temperature.
    Primary antibody incubated for 16 hours.

    See Abreview

  • ab57494 staining TAF7 in human bladder cancer tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using TEG. Samples were then blocked with 1% serum for 1 hour at room temperature followed by incubation with the primary antibody at a 1/3500 dilution for 1 hour. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody.

    See Abreview

Anti-TAF7 antibody (ab57494)参考文献

This product has been referenced in:
  • Gegonne A  et al. The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells. Mol Cell Biol 32:1984-97 (2012). WB . Read more (PubMed: 22411629) »
  • Devaiah BN & Singer DS Cross-talk Among RNA Polymerase II Kinases Modulates C-terminal Domain Phosphorylation. J Biol Chem 287:38755-66 (2012). WB . Read more (PubMed: 23027873) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (bladder cancer)
Specification bladder cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TEG
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. Karin Birkenkamp-Demtroeder

Verified customer

提交于 May 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (SW780 bladder cancer)
Loading amount 25 µg
Specification SW780 bladder cancer
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Karin Birkenkamp-Demtroeder

Verified customer

提交于 Apr 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"