概述

  • 产品名称Anti-TAF5抗体- ChIP Grade
    参阅全部 TAF5 一抗
  • 描述
    小鼠多克隆抗体to TAF5 - ChIP Grade
  • 经测试应用适用于: WB, ChIPmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Fusion protein:

    TWSCVVTYRGHVYPVWDVRFAPHGYYFVSCSYDKTARLWATDSNQALRVF VGHLSDVDCVQFHPNSNYVATGSSDRTVRLWDNMTGQSVRLMTGHKGSVS

    , corresponding to amino acids 481-580 of Drosophila melanogaster TAF5.

  • 常规说明

    Produced from outbred CD1 mice.

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液Constituent: 50% Glycerol
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 79 kDa.
ChIP Use at an assay dependent concentration. PubMed: 20097756

靶标

  • 相关性TAF5 is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It may play a role in helping to anchor Taf4 within the TFIID complex. It may be involved in transducing signals from various transcriptional regulators to the RNA polymerase II transcription machinery. TAF5 belongs to the TFIID complex which is composed of TATA binding protein (Tbp) and a number of TBP-associated factors (Tafs).
  • 细胞定位Nuclear
  • 别名
    • fc38g05 antibody
    • MGC123129 antibody
    • p85 antibody
    • TAF(II)100 antibody
    • TAF2D antibody
    • TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa antibody
    • TAFII 80 antibody
    • TAFII100 antibody
    • TATA box binding protein (TBP)-associated factor 2D antibody
    • TATA box binding protein (TBP)-associated factor, RNA polymerase II, D, 100kD antibody
    • TBP-associated factor, RNA polymerase II, 100-KD antibody
    • Transcription initiation factor TFIID 100 kDa subunit antibody
    • Transcription initiation factor TFIID 85 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 5 antibody
    • wu:fc38g05 antibody
    • zgc:123129 antibody
    see all

Anti-TAF5 antibody - ChIP Grade (ab21765)参考文献

This product has been referenced in:
  • Lee MC  et al. Drosophila octamer elements and Pdm-1 dictate the coordinated transcription of core histone genes. J Biol Chem 285:9041-53 (2010). ChIP ; Drosophila melanogaster . Read more (PubMed: 20097756) »

See 1 Publication for this product

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