概述

  • 产品名称
  • 描述
    兔多克隆抗体to TAF3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Guinea pig, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region from internal sequence amino acids 540-589 (

    RDREREKDKNKDKSKEKDKVKEKEKDKETGRETKYPWKEFLKEEEADPYK

    ) of Human TAF3 (NP_114129)

  • 阳性对照
    • HepG2 Cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 104 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.
  • 序列相似性
    Belongs to the TAF3 family.
    Contains 1 PHD-type zinc finger.
  • 结构域
    The PHD-type zinc finger mediates binding to histone H3 methyllysine at position 4 (H3K4me3).
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • 140 kDa TATA box-binding protein-associated factor antibody
    • Bip2 protein antibody
    • RNA polymerase II transcription factor TAFII140 antibody
    • TAF(II)140 antibody
    • TAF140 antibody
    • TAF3 antibody
    • TAF3 RNA polymerase II TATA box binding protein (TBP)-associated factor antibody
    • TAF3_HUMAN antibody
    • TAFII-140 antibody
    • TAFII140 antibody
    • TAFII155 protein antibody
    • TATA box binding protein associated factor 3 antibody
    • TBP-associated factor 3 antibody
    • Transcription initiation factor TFIID 140 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 3 antibody
    see all

Anti-TAF3 antibody 图像

  • Anti-TAF3 antibody (ab85419) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 Cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 104 kDa
    Observed band size : 92 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-TAF3 antibody (ab85419)参考文献

ab85419 has not yet been referenced specifically in any publications.

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