The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IHC-P: 1/100 - 1/500.
Antigen exposure is recommended.
Antigen exposure with citrate buffer will enhance staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
组织特异性Ubiquitous. Observed in all fetal and adult tissues.
疾病相关Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
序列相似性Belongs to the RRM TET family. Contains 1 RanBP2-type zinc finger. Contains 1 RRM (RNA recognition motif) domain.
翻译后修饰Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity. Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
ab84369 at 1/250, staining human TAF15 in a section of human stomach adenocarcinoma by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection: DAB staining using anti-rabbit antibody at 1/100.
Anti-TAF15 antibody (ab84369)参考文献
has not yet been referenced specifically in any publications.