概述

  • 产品名称Anti-TAF15抗体
    参阅全部 TAF15 一抗
  • 描述
    兔多克隆抗体to TAF15
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to region between residue 550 and the C-terminus (residue 592) of Human TAF15 (Swiss-Prot entry Q92804)

  • 阳性对照
    • Whole cell lysate from 293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab70390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 1 - 4 µg/mg of lysate.
    WB: 1/10000 - 1/30000. Detects a band of approximately 66 kDa (predicted molecular weight: 62 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
    • 组织特异性Ubiquitous. Observed in all fetal and adult tissues.
    • 疾病相关Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
    • 序列相似性Belongs to the RRM TET family.
      Contains 1 RanBP2-type zinc finger.
      Contains 1 RRM (RNA recognition motif) domain.
    • 翻译后修饰Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 68 kDa TATA-binding protein-associated factor antibody
      • Npl3 antibody
      • RBP56 antibody
      • RBP56_HUMAN antibody
      • RNA binding protein 56 antibody
      • RNA-binding protein 56 antibody
      • TAF antibody
      • TAF(II)68 antibody
      • TAF15 antibody
      • TAF15 RNA polymerase II TATA box binding protein (TBP) associated factor 68kDa antibody
      • TAF2N antibody
      • TAFII68 antibody
      • TATA-binding protein-associated factor 2N antibody
      see all

    Anti-TAF15 antibody 图像

    • Anti-TAF15 antibody (ab70390) at 1/20000 dilution + Whole cell lysate from 293T cells at 200 µg
      Developed using the ECL technique

      Predicted band size : 62 kDa
      Observed band size : 66 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 5 seconds

    Anti-TAF15 antibody (ab70390)参考文献

    ab70390 has not yet been referenced specifically in any publications.

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