概述

  • 产品名称Anti-TAF15抗体
    参阅全部 TAF15 一抗
  • 描述
    兔多克隆抗体to TAF15
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 175 and 225 of Human TAF15 (SwissProt Q92804)

  • 阳性对照
    • Whole cell lysate from 293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab70389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/20000. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
IP Use at 1-4 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
  • 组织特异性Ubiquitous. Observed in all fetal and adult tissues.
  • 疾病相关Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
  • 序列相似性Belongs to the RRM TET family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 68 kDa TATA-binding protein-associated factor antibody
    • Npl3 antibody
    • RBP56 antibody
    • RBP56_HUMAN antibody
    • RNA binding protein 56 antibody
    • RNA-binding protein 56 antibody
    • TAF antibody
    • TAF(II)68 antibody
    • TAF15 antibody
    • TAF15 RNA polymerase II TATA box binding protein (TBP) associated factor 68kDa antibody
    • TAF2N antibody
    • TAFII68 antibody
    • TATA-binding protein-associated factor 2N antibody
    see all

Anti-TAF15 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling TAF15 with ab70389 at 1/5000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-TAF15 antibody (ab70389) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from 293T cells at 200 µg
    Lane 2 : Whole cell lysate from 293T cells immunoprecipitated with rabbit IgG control.
    Lane 3 : Whole cell lysate from 293T cells immunoprecipitated with 0.3 µg/ml ab70389.


    Predicted band size : 62 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa (possible IgG),60 kDa (possible IgG).
    10 mg of Whole cell lysate from 293T cells was used for IP.

Anti-TAF15 antibody (ab70389)参考文献

ab70389 has not yet been referenced specifically in any publications.

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