The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/20000. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
Use at 1-4 µg/mg of lysate.
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
组织特异性Ubiquitous. Observed in all fetal and adult tissues.
疾病相关Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
序列相似性Belongs to the RRM TET family. Contains 1 RanBP2-type zinc finger. Contains 1 RRM (RNA recognition motif) domain.
翻译后修饰Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity. Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling TAF15 with ab70389 at 1/5000 (0.2µg/ml). Detection: DAB.
Western blot - TAF15 antibody (ab70389)
All lanes : Anti-TAF15 antibody (ab70389) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells
at 200 µg Lane 2 : Whole cell lysate from 293T cells immunoprecipitated with rabbit IgG control. Lane 3 : Whole cell lysate from 293T cells immunoprecipitated with 0.3 µg/ml ab70389.