Anti-TAF15抗体[4D71] (ab124746)

概述

  • 产品名称
    Anti-TAF15抗体[4D71]
    参阅全部 TAF15 一抗
  • 描述
    小鼠单克隆抗体[4D71] to TAF15
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Recombinant full length Human TAF15, expressed in and purified from E. coli.

  • 阳性对照
    • WB: NIH/3T3 and HeLa cell lysate. ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab124746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
ICC/IF 1/500 - 1/1000.

靶标

  • 功能
    RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
  • 组织特异性
    Ubiquitous. Observed in all fetal and adult tissues.
  • 疾病相关
    Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
  • 序列相似性
    Belongs to the RRM TET family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰
    Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 68 kDa TATA-binding protein-associated factor antibody
    • Npl3 antibody
    • RBP56 antibody
    • RBP56_HUMAN antibody
    • RNA binding protein 56 antibody
    • RNA-binding protein 56 antibody
    • TAF antibody
    • TAF(II)68 antibody
    • TAF15 antibody
    • TAF15 RNA polymerase II TATA box binding protein (TBP) associated factor 68kDa antibody
    • TAF2N antibody
    • TAFII68 antibody
    • TATA-binding protein-associated factor 2N antibody
    see all

图片

  • Lanes 2-5 : Anti-TAF15 antibody [4D71] (ab124746) at 1/1000 dilution

    Lane 1 : Protein standard
    Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell cytosol fraction
    Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) cell nuclear fraction
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell cytosol fraction
    Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell nuclear fraction

    Predicted band size: 62 kDa

  • Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for TAF15 using ab124746 (red) at a 1/500 dilution, and co-stained with rabbit anti-GAPDH (green) at a 1/1000 dilution. Hoechst was used for nuclear DNA staining (blue).

  • Anti-TAF15 antibody [4D71] (ab124746) at 1 µg/ml + Crude HeLa cell homogenate

    Predicted band size: 62 kDa

  • ab124746, at 1 µg/ml, staining TAF15 (green) in HeLa cell cultures by Immunofluorescence. Blue is a DNA stain and red is Vimentin.

文献

ab124746 has not yet been referenced specifically in any publications.

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