The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
Use a concentration of 1 - 5 µg/ml.
功能RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
组织特异性Ubiquitous. Observed in all fetal and adult tissues.
疾病相关Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
序列相似性Belongs to the RRM TET family. Contains 1 RanBP2-type zinc finger. Contains 1 RRM (RNA recognition motif) domain.
翻译后修饰Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity. Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.