概述

  • 产品名称Anti-TAF11抗体
    参阅全部 TAF11 一抗
  • 描述
    小鼠单克隆抗体to TAF11
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment: SKVFVGEVVE EALDVCEKWG EMPPLQPKHM REAVRRLKSK GQIPNSKHKK IIF, corresponding to amino acids 158-211 of Human TAF11

性能

应用

Our Abpromise guarantee covers the use of ab57501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 23 kDa.
IHC-P Use a concentration of 3 µg/ml.

靶标

  • 功能Core TAFII present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively).
  • 序列相似性Belongs to the TAF11 family.
  • 结构域TBP and TAFII18 bind to distinct domains of TAFII28.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC:15243 antibody
    • p30 beta antibody
    • PRO2134 antibody
    • TAF antibody
    • TAF(II)28 antibody
    • TAF11 antibody
    • TAF11 RNA polymerase II TATA box binding protein (TBP) associated factor 28 kD antibody
    • TAF11_HUMAN antibody
    • TAF2I antibody
    • TAFII 28 antibody
    • TAFII-28 antibody
    • TAFII28 antibody
    • TFIID subunit p30 beta antibody
    • TFIID subunit p30-beta antibody
    • Transcription initiation factor TFIID 28 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 11 antibody
    see all

Anti-TAF11 antibody 图像

  • ab57501 staining TAF11 in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton in PBS for 10 minutes and blocked with 5% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation in sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/500 in 0.5% Triton + PBS) for 16 hours at 4°C. A Cy3®-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • TAF11 antibody (ab57501) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.


  • Predicted band size : 23 kDa
    TAF11 antibody (ab57501) at 1ug/lane + HeLa cell lysate at 25ug/lane.

Anti-TAF11 antibody (ab57501)参考文献

This product has been referenced in:
  • Yao J  et al. Subnuclear segregation of genes and core promoter factors in myogenesis. Genes Dev 25:569-80 (2011). ICC/IF ; Mouse . Read more (PubMed: 21357673) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Na-cytrate pH 6
Sample Mouse Tissue sections (Liver)
Specification Liver
Permeabilization Yes - PBS-Triton 0,1% 10min
Fixative Paraformaldehyde
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Dr. Daniel Alpern

Verified customer

提交于 Apr 13 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"