Anti-TAF1抗体(ab188427)
Key features and details
- Rabbit polyclonal to TAF1
- Suitable for: ICC/IF, WB
- Reacts with: Human, Syrian hamster
- Isotype: IgG
概述
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产品名称
Anti-TAF1抗体
参阅全部 TAF1 一抗 -
描述
兔多克隆抗体to TAF1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human, Syrian hamster -
免疫原
Synthetic peptide corresponding to Human TAF1 aa 100-200 conjugated to keyhole limpet haemocyanin (Cysteine residue).
Database link: P21675 -
阳性对照
- HeLa whole cell extract; BHK and HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Preservative: 0.05% Sodium azide -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab188427于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/1000 - 1/5000.
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WB |
1/1000. Predicted molecular weight: 213 kDa.
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说明 |
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ICC/IF
1/1000 - 1/5000. |
WB
1/1000. Predicted molecular weight: 213 kDa. |
靶标
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相关性
TAF1 functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. Compared to higher eukaryotes TAF1, yeast TAF1 lacks the C-terminal bromodomains and C-terminal kinase activity. The TFIID interacting proteins BDF1 and BDF2 may substitute for these domains. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 6872 Human
- Omim: 313650 Human
- SwissProt: P21675 Human
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别名
- BA2R antibody
- CCG 1 antibody
- CCG1 antibody
see all
图片
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Immunofluorescence staining of TAF1 in HeLa cells with ab188427 at a 1/5000 dilution. Hela cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100.
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Anti-TAF1 antibody (ab188427) at 1/1000 dilution + HeLa whole cell extract at 10 µg
Predicted band size: 213 kDa -
IImmunofluorescence staining of TAF1 in in BHK cells (fixed with 3% formaldehyde and permiabilized 1% Nonidet P-40) with ab188427 at 1/1000 dilution. Secondary staining with Goat anti-Rabbit IgG conjugated with rhodamine.
Panel a): Stained with ab188427 without immunizing peptide.
Panel b): Stained with ab188427 with immunizing peptide.
Panel c): Panel a) co-stained with Hoechst 33258.
Panel d): Panel b) co-stained with Hoechst 33258.
Panel e): Phase-contrast image of panels a) and c).
Panel f): Phase-contrast image of panels b) and d).
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab188427 被引用在 2 文献中.
- Yu X et al. Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours. J Extracell Vesicles 11:e12185 (2022). PubMed: 35029057
- Sekiguchi T et al. The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein. Mol Cell Biol 11:3317-25 (1991). WB, ICC/IF . PubMed: 2038334