概述

  • 产品名称
    Anti-T7 tag®抗体(HRP)
    参阅全部 T7 tag® 一抗
  • 描述
    兔多克隆抗体to T7 tag® (HRP)
  • 偶联物
    HRP
  • 特异性
    Ab21477 recognises T7 tag.
  • 经测试应用
    适用于: ELISA, WB, ICCmore details
  • 种属反应性
    May react with Bordetella phage BPP-1, Yersinia pestis phage phiA1122, Bacteriophage T7, based on sequence homology. Not yet tested in other species.
  • 免疫原

    Synthetic peptide:

    MASMTGGQQMG

    conjugated to KLH, corresponding to amino acids 1-11 of T7 tag

  • 常规说明
    T7-TAG® is a trademark of EMD Biosciences, Inc.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, 50mM HEPES, 100mM Sodium chloride, pH 7.1
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 42 kDa.
ICC Use a concentration of 0.5 - 2 µg/ml.

靶标

  • 相关性
    The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • 别名
    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

Anti-T7 tag® antibody (HRP) (ab21477)参考文献

This product has been referenced in:

See 1 Publication for this product

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