概述

  • 产品名称Anti-T7 tag®抗体(Agarose)
    参阅全部 T7 tag® 一抗
  • 描述
    山羊多克隆抗体to T7 tag® (Agarose)
  • 偶联物Agarose
  • 经测试应用适用于: IPmore details
    不适用于: ChIP
  • 免疫原

    Goats were immunized with

    MASMTGGQQMG

    (T7) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected.

  • 常规说明T7-TAG® is a trademark of EMD Biosciences, Inc.

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

性能

应用

Our Abpromise guarantee covers the use of ab1230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.
  • 应用说明Is unsuitable for ChIP.
  • 靶标

    • 相关性The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
    • 别名
      • 10 antibody
      • Major capsid protein 10A antibody
      • Major capsid protein antibody
      • Minor capsid protein 10B antibody
      • T7 epitope tag antibody
      see all

    Anti-T7 tag® antibody (Agarose) (ab1230)参考文献

    This product has been referenced in:
    • Piccinni E  et al. Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours. Acta Biochim Pol 58:529-34 (2011). IP . Read more (PubMed: 22068108) »
    • MacPherson MJ  et al. The CTCF insulator protein is posttranslationally modified by SUMO. Mol Cell Biol 29:714-25 (2009). IP . Read more (PubMed: 19029252) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us.

    Indeed, we can confirm that Agarose-conjugated anti-T7 tag® antibodyab1230 can be used to purify (or pull-down) T7-tagged proteins, as it has been tested and is guaranteed to work in immunoprecipitation (IP). ...

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    Thank you for contacting us for technical support with ab1230 on behalf of your customer. Unfortunately it is very hard for me to understand the customer's protocol and results as limited information was provided. Can you please explain what the ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"