概述

  • 产品名称Anti-T7 tag®抗体
    参阅全部 T7 tag® 一抗
  • 描述
    山羊多克隆抗体to T7 tag®
  • 经测试应用适用于: ELISA, WB, ICC, IHC-Pmore details
  • 免疫原

    T7 (MASMTGGQQMG) coupled to KLH

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 0.03 - 10 µg/ml. Coating: 2-10 ug/ml; Primary: 0.03-1 ug/ml
WB Use a concentration of 0.03 - 1 µg/ml. Colorimetric: 0.1-1 ug/ml; Chemiluminescent: 0.03-1 ug/ml
ICC Use a concentration of 0.5 - 5 µg/ml.
IHC-P Use at an assay dependent dilution.

靶标

  • 相关性The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • 别名
    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

Anti-T7 tag® antibody 图像

  • All lanes : Anti-T7 tag® antibody (ab97964) at 0.4 µg/ml

    Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
    Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
    Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg

    Developed using the ECL technique

    Exposure time : 30 seconds

Anti-T7 tag® antibody (ab97964)参考文献

ab97964 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"