概述

  • 产品名称Anti-T7 tag®抗体
    参阅全部 T7 tag® 一抗
  • 描述
    兔多克隆抗体to T7 tag®
  • 特异性ELISA: The anti T7 diluted 1:176,000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification.
  • 经测试应用适用于: ICC/IF, ELISA, WBmore details
  • 免疫原

    MASMTGGQQMG

    (T7 epitope) conjugated to KLH.

  • 常规说明T7-TAG® is a trademark of EMD Biosciences, Inc.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液PBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support.
  • 克隆多克隆
  • 同种型unknown
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

靶标

  • 相关性The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • 别名
    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

Anti-T7 tag® antibody (ab9115)参考文献

This product has been referenced in:
  • Horn AV  et al. Human LINE-1 restriction by APOBEC3C is deaminase independent and mediated by an ORF1p interaction that affects LINE reverse transcriptase activity. Nucleic Acids Res N/A:N/A (2013). ICC/IF ; Human . Read more (PubMed: 24101588) »
  • Kubota T  et al. Virus infection triggers SUMOylation of IRF3 and IRF7, leading to the negative regulation of type I interferon gene expression. J Biol Chem 283:25660-70 (2008). WB . Read more (PubMed: 18635538) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

Ab9115 and ab18184 are provided at 1mg/ml. For both antibodies I would recommend an initial dilution 1/1000 and try serial dilutions in order to optimise it to your experiment.

You can also have a look a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela)
Specification Hela
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

提交于 Jan 27 2006

Thank you very much for those details, it is very useful. I am sorry you are not satisfied with ab9115. In this instance, without admission of responsibility or liability, I am prepared to offer you a credit note or refund for the value of the antib...

Read More

This antibody has not yet been tested in ICC. We have two further antibodies to T7 epitope tag (ab9112 an ab9138), but neither of these have been tested in ICC !

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"