T47D全细胞裂解液(ab14899)

概述

  • 产品名称T47D全细胞裂解液
    参阅全部 T47D 细胞裂解液
  • 常规说明Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.90
    Constituents: 0.48% HEPES, 0.75% Potassium chloride, 0.01% Magnesium chloride, 20% Glycerol, 0.009% PMSF, 0.008% DTT
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 研究领域
  • 背景T47D cells carry receptors for a variety of steroids and calcitonin. They express mutant tumor suppressor protein p53 protein. Under normal culturing conditions, they express progesterone receptor constitutively and are responsive to estrogen. They are able to lose the estrogen receptor (ER) during long-term estrogen deprivation in vitro. Cells are therefore used as a model for studies of drug resistance to tamoxifen in patients with mutant p53 breast tumors.

T47D whole cell lysate (ab14899)参考文献

This product has been referenced in:
  • Haglund F  et al. Prolactin receptor in primary hyperparathyroidism - expression, functionality and clinical correlations. PLoS One 7:e36448 (2012). Read more (PubMed: 22606260) »

See 1 Publication for this product

Product Wall

Regarding ab14899, the procedure for the preparation of the whole cell extract is not RNAase free so the RNA will be at least partially degraded. But there is no step to get rid of the RNA and since it’s more soluble than DNA it could be present ...

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Thank you for your enquiry. With the protocol used, the DNA is pelleted and discarded along with cell debris during the extraction process, so there is no DNA present in this lysate. I hope this information helps. Please do not hesitate to conta...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"