概述

  • 产品名称Anti-T Plastin抗体
    参阅全部 T Plastin 一抗
  • 描述
    兔多克隆抗体to T Plastin
  • 特异性This antibody is specific for T Plastin.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to the N-terminal of human T Plastin.

  • 阳性对照
    • T plastin expressing bacterial extract, Sezary cells

性能

应用

Our Abpromise guarantee covers the use of ab45769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 70 kDa.
ELISA 1/2000 - 1/5000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.
  • 组织特异性Expressed in a variety of organs, including muscle, brain, uterus and esophagus.
  • 序列相似性Contains 2 actin-binding domains.
    Contains 4 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BMND18 antibody
    • Plastin 3 (T isoform) antibody
    • Plastin 3 antibody
    • Plastin-3 antibody
    • PLS3 antibody
    • PLST_HUMAN antibody
    • T fimbrin antibody
    • T-plastin antibody
    see all

Anti-T Plastin antibody 图像

  • All lanes : Anti-T Plastin antibody (ab45769) at 1/1000 dilution

    Lane 1 : Bacterial extract expressing T-Plastin at 1 µg
    Lane 2 : Bacterial extract expressing L-Plastin at 1 µg
    Lane 3 : Bacterial extract expressing I-Plastin at 1 µg
    Lane 4 : Sezary cell extract at 10 µg
    Lane 5 : Control T-Helper cells at 10 µg


    Predicted band size : 70 kDa
    Observed band size : 70 kDa
  • IHC image of ab45769 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45769, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-T Plastin antibody (ab45769)参考文献

This product has been referenced in:
  • Jones CL  et al. Regulation of T-Plastin Expression by Promoter Hypomethylation in Primary Cutaneous T-Cell Lymphoma. J Invest Dermatol : (2012). Human . Read more (PubMed: 22495182) »
  • Bollinger KE  et al. Proteomic similarities in steroid responsiveness in normal and glaucomatous trabecular meshwork cells. Mol Vis 18:2001-11 (2012). WB ; Human . Read more (PubMed: 22876128) »

See all 2 Publications for this product

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